Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.
J Biotechnol. 2012 Jan;157(1):38-49. doi: 10.1016/j.jbiotec.2011.10.014. Epub 2011 Nov 11.
Avidins represent an interesting group of proteins showing high structural similarity and ligand-binding properties but low similarity in primary structure. In this study, we show that it is possible to create functional chimeric proteins from the avidin protein family when applying DNA family shuffling to the genes of the avidin protein family: avidin, avidin related gene 2 and biotin-binding protein A. The novel chimeric proteins were selected by phage display biopanning against biotin, and the selected enriched proteins were characterized, displaying diverse features distinct from the parental genes, including binding to cysteine.
avidins 是一组具有高度结构相似性和配体结合特性的有趣蛋白质，但在一级结构上的相似性较低。在这项研究中，我们通过对 avidin 蛋白家族的基因进行 DNA 家族改组，展示了从 avidin 蛋白家族中创建功能性嵌合蛋白的可能性：avidin、avidin 相关基因 2 和生物素结合蛋白 A。新型嵌合蛋白通过噬菌体展示生物淘选针对生物素进行选择，对所选富集蛋白进行了表征，显示出与亲本基因不同的多种特征，包括与半胱氨酸结合。
