Ren Xian-pei, Liu Gang, Zhou Zai-jin, Liu Fei, Li Zhi-yong, Song Li-liz
Department of Physics, Yunnan Normal University, Kunming 650092, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Aug;30(8):2120-3.
Fourier transform infrared spectroscopy (FTIR) was used to study three group tobacco leaves of brown spot, angular spot and weather speck, with each group being composed of three samples, namely, leaf spots, near-spot and normal tobacco leaves. The results indicate that the absorption ratio A1631/A1025 of the three group tobacco leaves showed the same change tendency, with the normal tobacco leaves < the near-spot leaves < the leaf spots. For a more objective and comprehensive analysis, the original and second-derivative spectra were selected for distance analysis in the whole region. The results show that the Pearson correlation coefficient of the near-spot leaves and normal leaves is greater than the corresponding coefficient of leaf spots and normal leaves, which suggest that the near-spot leaves and normal leaves have a closer relationship compared with the leaf spots and normal leaves. The ratios of the A1631/A1025 and Pearson correlation coefficients show that the chemical composition of the near-spot leaves changed gradually, that is, the near-spot leaves were in a transient state between normal and disease leaves. In conclusion, FTIR spectroscopy is a promising technique for diagnosing tobacco disease in the incubation period.
采用傅里叶变换红外光谱（FTIR）对褐斑病、角斑病和气候斑三种类型的烟叶进行研究，每组由三个样本组成，即病斑叶、病斑附近叶和正常烟叶。结果表明，三组烟叶的A1631/A1025吸收比值呈现相同的变化趋势，即正常烟叶＜病斑附近叶＜病斑叶。为了进行更客观全面的分析，选取原始光谱和二阶导数光谱在全区域进行距离分析。结果显示，病斑附近叶与正常叶的皮尔逊相关系数大于病斑叶与正常叶的相应系数，这表明病斑附近叶与正常叶的关系比病斑叶与正常叶更为密切。A1631/A1025比值和皮尔逊相关系数表明，病斑附近叶的化学成分在逐渐变化，即病斑附近叶处于正常叶和病叶之间的过渡状态。综上所述，FTIR光谱技术在诊断烟草潜伏期病害方面具有广阔的应用前景。
