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[利用傅里叶变换红外光谱法对稻瘟病、玉米和蚕豆锈病叶片的研究]

[Research on rice blast, corn and broad bean rust leaves by FTIR spectroscopy].

作者信息

Ou Quan-Hong, Zhao Xing-Xiang, Zhou Xiang-Ping, Liu Gang, Li Lun, Zhang Li

机构信息

School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2012 Sep;32(9):2389-92.

PMID:23240403
Abstract

Fourier transform infrared Spectroscopy (FTIR) was used to study healthy and diseased leaves of rice, corn, and broad bean. The results show that the infrared spectra of the leaves are mainly composed of the absorption bands of proteins and polysaccharide. The spectra are similar, but the differences were observed in the peak positions, shapes, and absorption ratios. The remarkable differences in correlation coefficients were also observed in the region of 1 800-1 000 cm(-1) of the second derivate spectra. The absorption ratio A1 056 /A1 652 of healthy rice leaves and rice blast leaves, A1 652/A2 920 of healthy broad bean leaves and leaves, A1 056/A2 920 of healthy corn and corn rust (non-lesion site), and A1 652/A2 920 of healthy corn and corn rust (lesion site) all show a decline from healthy to diseased leaves, showing that the contents of polysaccharide and the protein have some differences.

摘要

傅里叶变换红外光谱(FTIR)被用于研究水稻、玉米和蚕豆的健康叶片与患病叶片。结果表明,叶片的红外光谱主要由蛋白质和多糖的吸收带组成。光谱相似,但在峰位、形状和吸收比方面存在差异。在二阶导数光谱的1800 - 1000 cm⁻¹区域也观察到相关系数的显著差异。健康水稻叶片与稻瘟病叶片的吸收比A1056 / A1652、健康蚕豆叶片与患病叶片的A1652 / A2920、健康玉米与玉米锈病(非病斑部位)的A1056 / A2920以及健康玉米与玉米锈病(病斑部位)的A1652 / A2920均显示从健康叶片到患病叶片呈下降趋势,表明多糖和蛋白质的含量存在一些差异。

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