Vector Control Research Centre (Indian Council of Medical Research), Medical Complex, Indira Nagar, Puducherry 605 006, India.
J Med Entomol. 2010 Sep;47(5):743-7. doi: 10.1603/me09195.
Species-specific differences encountered in the nucleotide sequences of a highly variable region of the 18S rRNA gene were used to design a multiplex polymerase chain reaction (PCR) assay for the identification of Phlebotomus papatasi Scopoli and Phlebotomus argentipes An-nandale & Brunetti, vectors of Leishmania. This multiplex PCR assay uses a common forward primer and two reverse primers, which are specific for the two species. Amplification of a PCR product of size 788 bp indicates the presence of P. papatasi, whereas a product of size 677 bp indicates the presence of P. argentipes. The assay was found to be highly specific and sensitive.
利用 18S rRNA 基因高度可变区的核苷酸序列中出现的种特异性差异，设计了一种多重聚合酶链反应（PCR）检测方法，用于鉴定利什曼原虫的传播媒介白蛉属 Papatasi 和白蛉属 Argentipes。该多重 PCR 检测方法使用一个通用的正向引物和两个特异性针对这两个物种的反向引物。大小为 788 bp 的 PCR 产物的扩增表明存在 Papatasi，而大小为 677 bp 的产物表明存在 Argentipes。该检测方法具有高度的特异性和敏感性。
