Wilken C, Van Kirk E A, Slaughter R G, Ji T H, Murdoch W J
Department of Animal Science, University of Wyoming, Laramie 82071.
Prostaglandins. 1990 Dec;40(6):637-46. doi: 10.1016/0090-6980(90)90008-j.
Thromboxane (TX) B2, a stable metabolic product of hydrolysis of TXA2, was measured by radioimmunoassay in tissue extracts of ovaries of immature rats pretreated with pregnant mare's serum gonadotropin and human chorionic gonadotropin. Ovarian concentrations of TXB2 increased before, and remained elevated after, the time of ovulation. In a subsequent study, ovulation was inhibited in a dose-dependent fashion by a reported TXA2 receptor antagonist, AH23848. Nevertheless, inhibition of the preovulatory rise in synthesis of TXB2 by furegrelate (a thromboxane synthetase inhibitor) did not prevent ovulation. Nor was the blockade of ovulation caused by indomethacin (a cyclooxygenase inhibitor) reversed by a TXA2 mimetic (U-46619). It does not appear that a preovulatory increase in ovarian thromboxane is an obligatory component of the ovulatory mechanism of gonadotropin-primed immature rats.
血栓素（TX）B2是血栓素A2水解后的稳定代谢产物，采用放射免疫分析法在经孕马血清促性腺激素和人绒毛膜促性腺激素预处理的未成熟大鼠卵巢组织提取物中进行测定。排卵前，卵巢中TXB2的浓度升高，排卵后仍保持在较高水平。在随后的一项研究中，一种报道的TXA2受体拮抗剂AH23848以剂量依赖的方式抑制排卵。然而，呋格雷酯（一种血栓素合成酶抑制剂）对排卵前TXB2合成增加的抑制作用并未阻止排卵。吲哚美辛（一种环氧化酶抑制剂）引起的排卵阻断也不能被TXA2模拟物（U-46619）逆转。在促性腺激素预处理的未成熟大鼠排卵机制中，卵巢血栓素排卵前增加似乎并非必需成分。
