Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Stomatology Hospital, Sichuan University, 14#, 3rd Section, Renmin Nan Road, Chengdu 610041, PR China.
J Dent Res. 2011 Jan;90(1):115-20. doi: 10.1177/0022034510385237. Epub 2010 Oct 12.
There is increasing interest in the development of new in vitro tissue models. In this study, a tissue model of periodontal ligament (PDL) was established by 3-D-culturing human PDL cells in a thin sheet of porous poly (lactic-co-glycolic acid) scaffold. Growth of the model was evidenced by MTT assay and various microscopies. After being subjected to static compression of 5 ~ 35 g/cm(2) for 6 hrs, the RANKL mRNA expression was significantly up-regulated by force ≥ 25 g/cm(2) in the model. After being subjected to static compression of 25 g/cm(2) for 6 ~ 72 hrs, the mRNA expression of PTHrP, IL-11, IL-8, and FGF-2, potential osteoclastogenesis inducers, was significantly up-regulated in the model, which was further verified by the compression of human PDL in vivo. However, when human gingival fibroblasts were substituted for PDL cells in the model, almost no osteoclastogenesis inducers were up-regulated by compression. This tissue model can serve as an effective tool for the study of PDL mechanoresponse.
periodontal ligament, PDL; periodontal ligament cells, PDLCs; poly (lactic-co-glycolic acid), PLGA; orthodontic tooth movement, OTM; extracellular matrix, ECM.
人们对开发新的体外组织模型越来越感兴趣。在这项研究中，通过将人牙周韧带细胞（PDLCs）三维培养在薄的多孔聚（乳酸-共-乙醇酸）（PLGA）支架中，建立了牙周韧带（PDL）组织模型。通过 MTT 测定法和各种显微镜观察，证明了模型的生长。在模型中，经过 535g/cm(2)的静态压缩 6 小时后，力≥25g/cm(2)可显著上调 RANKLmRNA 的表达。在模型中，经过 25g/cm(2)的静态压缩 672 小时后，PTHrP、IL-11、IL-8 和 FGF-2 的 mRNA 表达显著上调，这些基因可能是破骨细胞分化的诱导剂，这一结果通过体内人牙周韧带的压缩实验得到进一步验证。然而，当模型中的人牙龈成纤维细胞替代牙周韧带细胞时，压缩几乎不会上调任何破骨细胞分化诱导剂。该组织模型可作为研究牙周韧带力学反应的有效工具。
牙周韧带，PDL；牙周韧带细胞，PDLCs；聚（乳酸-共-乙醇酸），PLGA；正畸牙齿移动，OTM；细胞外基质，ECM。
