Department of Surgery, St. Vincent's University Hospital, UCD School of Medicine and Medical Sciences, University College Dublin, Dublin, Ireland.
Dis Colon Rectum. 2010 Nov;53(11):1530-6. doi: 10.1007/DCR.0b013e3181f1e620.
Debate persists regarding the role of Desulfovibrio subspecies in ulcerative colitis. Combined microscopic and molecular techniques enable this issue to be investigated by allowing precise enumeration of specific bacterial species within the colonic mucous gel. The aim of this study was to combine laser capture microdissection and quantitative polymerase chain reaction to determine Desulfovibrio copy number in crypt-associated mucous gel in health and in acute and chronic ulcerative colitis.
Colonic mucosal biopsies were harvested from healthy controls (n = 19) and patients with acute (n = 10) or chronic (n = 10) ulcerative colitis. Crypt-associated mucous gel was obtained by laser capture microdissection throughout the colon. Pan-bacterial 16S rRNA and Desulfovibrio copy number/mm were obtained by polymerase chain reaction at each locus. Bacterial copy numbers were interrogated for correlation with location and disease activity. Data were evaluated using a combination of ordinary linear methods and linear mixed-effects models to cater for multiple interactions.
Desulfovibrio positivity was significantly increased in acute and chronic ulcerative colitis at multiple levels within the colon, and after normalization with total bacterial signal, the relative Desulfovibrio load was increased in acute colitis compared with controls. Desulfovibrio counts did not significantly correlate with age, disease duration, or disease activity but interlevel correlations were found in adjacent colonic segments in the healthy control and chronic ulcerative colitis groups.
The presence of Desulfovibrio subspecies is increased in ulcerative colitis and the data presented suggest that these bacteria represent an increased percentage of the colonic microbiome in acute ulcerative colitis.
关于脱硫弧菌亚种在溃疡性结肠炎中的作用，一直存在争议。结合显微镜和分子技术，可以通过精确计数结肠黏液凝胶中特定细菌物种来研究这个问题。本研究的目的是结合激光捕获显微切割和定量聚合酶链反应来确定健康和急性及慢性溃疡性结肠炎患者隐窝相关黏液凝胶中脱硫弧菌的拷贝数。
从健康对照者（n=19）和急性（n=10）或慢性（n=10）溃疡性结肠炎患者中采集结肠黏膜活检。通过激光捕获显微切割获得整个结肠的隐窝相关黏液凝胶。在每个部位通过聚合酶链反应获得泛细菌 16S rRNA 和脱硫弧菌拷贝数/mm。用普通线性方法和线性混合效应模型的组合来检验细菌拷贝数与位置和疾病活动度的相关性，以适应多种相互作用。
在急性和慢性溃疡性结肠炎中，脱硫弧菌在结肠的多个部位呈阳性，并且在与总细菌信号归一化后，与对照组相比，急性结肠炎中相对脱硫弧菌负荷增加。脱硫弧菌计数与年龄、疾病持续时间或疾病活动度无显著相关性，但在健康对照组和慢性溃疡性结肠炎组相邻结肠节段中发现了水平间相关性。
溃疡性结肠炎中脱硫弧菌亚种的存在增加，并且所提出的数据表明，这些细菌在急性溃疡性结肠炎中代表了结肠微生物组的增加比例。
