Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, NJ 07103, USA
Anticancer Res. 2010 Sep;30(9):3629-35.
We observed previously that quercetin can increase the activity of the differentiation markers alkaline phosphatase and dipeptidyl peptidase in Caco-2 colon cancer cells. In the present work, we compared the effects of quercetin on cell proliferation and differentiation with the action of related flavonols and quercetin glycosides. Relative to the action of quercetin, effects on growth and enzyme activities did not always follow parallel trends but quercetin 3-glucoside was notably more potent in both respects while quercetin rutinoside was less active. Of the compounds examined, baicalein and myricetin caused the greatest production of hydrogen peroxide when incubated with the medium. Flavonols can have pro-oxidant effects, but our data suggested that this action was not the sole determinant of growth inhibitory or differentiating effects on Caco-2 cells. Our data indicated that effects of quercetin on colon cancer cell lines can be greatly affected by glycoside modification.
我们之前观察到槲皮素可以增加 Caco-2 结肠癌细胞分化标志物碱性磷酸酶和二肽基肽酶的活性。在本工作中，我们比较了槲皮素对细胞增殖和分化的作用与相关类黄酮和槲皮素糖苷的作用。相对于槲皮素的作用，对生长和酶活性的影响并不总是遵循平行的趋势，但槲皮素 3-葡萄糖苷在这两方面都明显更有效，而槲皮素鼠李糖苷的活性较低。在所检查的化合物中，黄芩素和杨梅素在与培养基孵育时产生的过氧化氢最多。类黄酮可能具有促氧化作用，但我们的数据表明，这种作用不是对 Caco-2 细胞的生长抑制或分化作用的唯一决定因素。我们的数据表明，糖苷修饰极大地影响了槲皮素对结肠癌细胞系的作用。
