Dihal Ashwin A, Woutersen Ruud A, van Ommen Ben, Rietjens Ivonne M C M, Stierum Rob H
Physiological Sciences Department, TNO Quality of Life, Zeist, The Netherlands.
Cancer Lett. 2006 Jul 18;238(2):248-59. doi: 10.1016/j.canlet.2005.07.007. Epub 2005 Aug 29.
The effect of the dietary flavonoid quercetin was investigated on proliferation and differentiation of the human colon cancer cell line Caco-2. Confluent Caco-2 monolayers exposed to quercetin showed a biphasic effect on cell proliferation and a decrease in cell differentiation (0.001<P<0.05). During differentiation Caco-2 cells formed 5 phase II metabolites, of which the amount of 4'-O-methyl-quercetin-3'-O-glucuronide correlated with the differentiation grade (r=0.99, P<0.003). The increment of cell proliferation at low quercetin concentrations and the decrease in cell differentiation are effects opposite to what would be expected for a functional food ingredient with anti-carcinogenic potential.
研究了膳食类黄酮槲皮素对人结肠癌细胞系Caco-2增殖和分化的影响。暴露于槲皮素的汇合Caco-2单层细胞对细胞增殖呈现双相效应，且细胞分化降低（0.001<P<0.05）。在分化过程中，Caco-2细胞形成5种II期代谢产物，其中4'-O-甲基槲皮素-3'-O-葡萄糖醛酸苷的量与分化程度相关（r=0.99，P<0.003）。低槲皮素浓度下细胞增殖的增加以及细胞分化的降低，这些效应与具有抗癌潜力的功能性食品成分的预期效应相反。
