Department of Pediatrics, China Medical University Hospital, Taichung, Taiwan, ROC.
Anticancer Res. 2010 Sep;30(9):3649-53.
The relationship between COX-2 gene and childhood leukemia risk is ambiguous. In this study, the association of genotypic polymorphisms in cyclooxygenase 2 (Cox-2) with childhood leukemia were investigated.
A total of 266 patients with childhood leukemia and 266 healthy controls recruited from the China Medical Hospital in central Taiwan were genotyped by PCR-RFLP method. Six polymorphic variants of Cox-2 were investigated, including G-1195A, G-765C, T+8473C, intron 1, intron 5, and intron 6, and the associations of specific genotypes with susceptibility to childhood leukemia were analysed.
The data showed that although there was no difference in the distribution for each genotype of Cox-2 G-1195A, G-765C, T+8473C, intron 1, intron 5, and intron 6, between the childhood leukemia and control groups (p>0.05), the analysis of combined effect for COX-2 G-765C and intron 6 showed that individuals with GC at G-765C and GG or AG+AA at intron 6 present a slightly higher potential for developing childhood leukemia than other groups.
These findings suggest that the C allele of COX-2 G-765C may be responsible for childhood leukemia and may be useful in early detection of child leukemia.
COX-2 基因与儿童白血病风险之间的关系尚不清楚。本研究旨在探讨环氧化酶 2（COX-2）基因多态性与儿童白血病的关系。
采用 PCR-RFLP 方法对来自中国台湾中部某医院的 266 例儿童白血病患者和 266 例健康对照者的 COX-2 基因进行基因分型。共检测了 COX-2 的 6 种多态性变异，包括 G-1195A、G-765C、T+8473C、内含子 1、内含子 5 和内含子 6，并分析了特定基因型与儿童白血病易感性的关系。
数据显示，虽然 Cox-2 G-1195A、G-765C、T+8473C、内含子 1、内含子 5 和内含子 6 各基因型在儿童白血病组和对照组之间的分布无差异（p＞0.05），但 COX-2 G-765C 和内含子 6 联合作用的分析表明，G-765C 处 GC 基因型和内含子 6 处 GG 或 AG+AA 基因型个体发生儿童白血病的潜在风险略高。
这些发现提示 COX-2 G-765C 的 C 等位基因可能与儿童白血病有关，可能有助于儿童白血病的早期检测。
