Krishna S Sri, Aravind L, Bakolitsa Constantina, Caruthers Jonathan, Carlton Dennis, Miller Mitchell D, Abdubek Polat, Astakhova Tamara, Axelrod Herbert L, Chiu Hsiu Ju, Clayton Thomas, Deller Marc C, Duan Lian, Feuerhelm Julie, Grant Joanna C, Han Gye Won, Jaroszewski Lukasz, Jin Kevin K, Klock Heath E, Knuth Mark W, Kumar Abhinav, Marciano David, McMullan Daniel, Morse Andrew T, Nigoghossian Edward, Okach Linda, Reyes Ron, Rife Christopher L, van den Bedem Henry, Weekes Dana, Xu Qingping, Hodgson Keith O, Wooley John, Elsliger Marc André, Deacon Ashley M, Godzik Adam, Lesley Scott A, Wilson Ian A
Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park,California, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1160-6. doi: 10.1107/S1744309110002514. Epub 2010 Mar 5.
SSO2064 is the first structural representative of PF01796 (DUF35), a large prokaryotic family with a wide phylogenetic distribution. The structure reveals a novel two-domain architecture comprising an N-terminal, rubredoxin-like, zinc ribbon and a C-terminal, oligonucleotide/oligosaccharide-binding (OB) fold domain. Additional N-terminal helical segments may be involved in protein-protein interactions. Domain architectures, genomic context analysis and functional evidence from certain bacterial representatives of this family suggest that these proteins form a novel fatty-acid-binding component that is involved in the biosynthesis of lipids and polyketide antibiotics and that they possibly function as acyl-CoA-binding proteins. This structure has led to a re-evaluation of the DUF35 family, which has now been split into two entries in the latest Pfam release (v.24.0).
SSO2064是PF01796（DUF35）的首个结构代表，PF01796是一个大型原核生物家族，具有广泛的系统发育分布。该结构揭示了一种新颖的双结构域架构，包括一个N端类红素氧还蛋白锌带和一个C端寡核苷酸/寡糖结合（OB）折叠结构域。额外的N端螺旋片段可能参与蛋白质-蛋白质相互作用。该家族某些细菌代表的结构域架构、基因组背景分析和功能证据表明，这些蛋白质形成了一种新型脂肪酸结合成分，参与脂质和聚酮类抗生素的生物合成，并且它们可能作为酰基辅酶A结合蛋白发挥作用。这一结构导致了对DUF35家族的重新评估，在最新的Pfam版本（v.24.0）中，该家族现已被拆分为两个条目。
