Denison Michael S, Rogers Jane M, Rushing S Renee, Jones Carol L, Tetangco Selwyna C, Heath-Pagliuso Sharon
University of California, Davis, Davis, California, USA.
Curr Protoc Toxicol. 2002 May;Chapter 4:Unit4.8. doi: 10.1002/0471140856.tx0408s11.
Analysis of the Ah Receptor Signal Transduction Pathway (Michael S. Denison, Jane M. Rohers, S. Renee Rushing. Carol L. Jones, Selwyna C. Tetangico, and Sharon Heath-Pagliuso, University of California, Davis, California).The protocols in this unit will allow researchers to detect the Ah receptor and characterize its functional activities (i.e., ligand binding, transformation and DNA binding, and gene expression) in their biological test system and to use these methods to detect chemical and biochemical events that affect this signaling system.
芳烃受体信号转导途径分析(迈克尔·S·丹尼森、简·M·罗尔斯、S·蕾妮·拉辛、卡罗尔·L·琼斯、塞尔维娜·C·特坦吉科和莎伦·希思 - 帕廖索,加利福尼亚大学戴维斯分校,加利福尼亚)。本单元中的实验方案将使研究人员能够在其生物测试系统中检测芳烃受体并表征其功能活性(即配体结合、转化和DNA结合以及基因表达),并使用这些方法检测影响该信号系统的化学和生化事件。