Dept. of Biotechnology, Lund University, SE-22200 Lund, Sweden.
Biotechnol Prog. 2010 Sep-Oct;26(5):1295-302. doi: 10.1002/btpr.422.
Applications of IDA in, for example, immobilized metal ion affinity chromatography for purification of His-tagged proteins are well recognized. The use of IDA as an efficient chelating adsorbent for environmental separations, that is, for the capture of heavy metals, is not studied. Adsorbents based on supermacroporous gels (cryogels) bearing metal chelating functionalities (IDA residues and ligand derived from derivatization of epoxy-cryogel with tris(2-aminoethyl)amine followed by the treatment with bromoacetic acid (defined as TBA ligand)) have been prepared and evaluated on capture of heavy metal ions. The cryogels were prepared in plastic carriers, resulting in desired mechanical stability and named as macroporous gel particles (MGPs). Sorption and desorption experiments for different metals (Cu²+, Zn²+, Cd²+, and Ni²+ with IDA adsorbent and Cu²+ and Zn²+ with TBA adsorbent) were carried out in batch and monolithic modes, respectively. Obtained capacities with Cu²+ were 74 μmol/mL (TBA) and 19 μmol/mL gel (IDA). The metal removal was higher for pH values between pH 3 and 5. Both adsorbents showed improved sorption at lower temperatures (10°C) than at higher (40°C) and the adsorption significantly dropped for the TBA adsorbent and Zn²+ at 40°C. Desorption of Cu²+ by using 1 M HCl and 0.1 M EDTA was successful for the IDA adsorbent whereas the desorption with the TBA adsorbent needs further attention. The result of this work has demonstrated that MGPs are potential treatment alternatives within the field of environmental separations and the removal of heavy metals from water effluents.
IDA 在固定化金属离子亲和层析中用于纯化 His 标记蛋白等方面的应用已得到广泛认可。然而，IDA 作为一种有效的螯合吸附剂在环境分离中的应用，即用于捕获重金属，尚未得到研究。本文制备了基于带有金属螯合功能的超大孔凝胶（冷冻凝胶）的吸附剂（IDA 残基和衍生自环氧冷冻凝胶与三（2-氨基乙基）胺的衍生化，然后用溴乙酸处理（定义为 TBA 配体）），并用于捕获重金属离子。冷冻凝胶在塑料载体中制备，以获得所需的机械稳定性，并将其命名为大孔凝胶颗粒（MGPs）。在分批和整体模式下分别进行了不同金属（Cu²+、Zn²+、Cd²+和 Ni²+与 IDA 吸附剂和 Cu²+和 Zn²+与 TBA 吸附剂）的吸附和解吸实验。用 Cu²+获得的容量分别为 74 μmol/mL（TBA）和 19 μmol/mL 凝胶（IDA）。金属去除率在 pH 值为 3 至 5 之间较高。两种吸附剂在较低温度（10°C）下的吸附性能均有所提高，而在较高温度（40°C）下则有所下降，并且在 40°C 时 TBA 吸附剂和 Zn²+的吸附明显下降。IDA 吸附剂可以用 1 M HCl 和 0.1 M EDTA 成功地解吸 Cu²+，而 TBA 吸附剂的解吸需要进一步关注。这项工作的结果表明，MGPs 是环境分离领域中潜在的重金属废水处理替代方案。
