USDA and North Carolina State University, Raleigh, North Carolina 27695, USA.
J Microsc. 2010 Nov;240(2):122-9. doi: 10.1111/j.1365-2818.2010.03393.x.
Construction of three-dimensional volumes from a series of two-dimensional images has been restricted by the limited capacity to decrease the opacity of tissue. The use of commercial software that allows colour-keying and manipulation of two-dimensional images in true three-dimensional space allowed us to construct three-dimensional volumes from pixel-based images of stained plant and animal tissue without generating vector information. We present three-dimensional volumes of (1) the crown of an oat plant showing internal responses to a freezing treatment, (2) a sample of a hepatocellular carcinoma from a woodchuck liver that had been heat-treated with computer-guided radiofrequency ablation to induce necrosis in the central portion of the tumour, and (3) several features of a sample of mouse lung. The technique is well suited to images from large sections (greater than 1 mm) generated from paraffin-embedded tissues. It is widely applicable, having potential to recover three-dimensional information at virtually any resolution inherent in images generated by light microscopy, computer tomography, magnetic resonance imaging or electron microscopy.
从一系列二维图像构建三维体积一直受到组织透明度降低能力的限制。使用允许对二维图像进行颜色键控和真正三维空间操作的商业软件，我们无需生成矢量信息，即可从染色的植物和动物组织的基于像素的图像构建三维体积。我们展示了（1）经过冷冻处理的燕麦植物冠部的三维体积，显示了内部反应，（2）经过计算机引导的射频消融热疗处理的土拨鼠肝脏中的肝细胞癌样本，以诱导肿瘤中央部分坏死，以及（3）来自小鼠肺部的几个样本的三维体积。该技术非常适合从石蜡包埋组织中生成的较大切片（大于 1 毫米）的图像。它具有广泛的适用性，具有在通过光学显微镜、计算机断层扫描、磁共振成像或电子显微镜生成的图像中几乎任何固有分辨率恢复三维信息的潜力。
