Livingston David P, Tuong Tan D
USDA-ARS and North Carolina State University, 840 Method Road, Unit 3, Raleigh, NC, 27695, USA,
Methods Mol Biol. 2014;1166:117-37. doi: 10.1007/978-1-4939-0844-8_11.
Histological analysis of frozen and thawed plants has been conducted for many years but the observation of individual sections provides only a 2-dimensional representation of a 3-dimensional phenomenon. Most techniques for viewing internal plant structure in three dimensions are either low in resolution or the instrument cannot penetrate deep enough into the tissue to visualize the whole plant. Techniques with higher resolution are expensive and equipment often requires time-consuming training. We present a relatively simple and less-expensive technique using pixel-based (JPEG) images of histological sections of an Arabidopsis thaliana plant and commercially available software to generate 3D reconstructions of internal structures. The technique has proven to work just as effectively for images from medical histology.
对冷冻和解冻植物的组织学分析已经进行了很多年，但对单个切片的观察仅提供了三维现象的二维呈现。大多数用于三维观察植物内部结构的技术要么分辨率低，要么仪器无法深入组织足够深度以可视化整个植物。分辨率较高的技术成本高昂，而且设备通常需要耗时的培训。我们提出了一种相对简单且成本较低的技术，该技术使用拟南芥植物组织切片的基于像素的（JPEG）图像和市售软件来生成内部结构的三维重建。事实证明，该技术对医学组织学图像同样有效。
