将遗传学和分子生物学应用于人类病原体新型隐球菌的研究。
Applying genetics and molecular biology to the study of the human pathogen Cryptococcus neoformans.
作者信息
Chun Cheryl D, Madhani Hiten D
机构信息
University of California, San Francisco, California, USA.
出版信息
Methods Enzymol. 2010;470:797-831. doi: 10.1016/S0076-6879(10)70033-1. Epub 2010 Mar 1.
Abstract
The basidiomycete yeast Crytococcus neoformans is a prominent human pathogen. It primarily infects immunocompromised individuals producing a meningoencephalitis that is lethal if untreated. Recent advances in its genetics and molecular biology have made it a model system for understanding both the Basidiomycota phylum and mechanisms of fungal pathogenesis. The relative ease of experimental manipulation coupled with the development of murine models for human disease allow for powerful studies in the mechanisms of virulence and host responses. This chapter introduces the organism and its life cycle and then provides detailed step-by-step protocols for culture, manipulation of the genome, analysis of nucleic acids and proteins, and assessment of virulence and expression of virulence factors.
摘要
担子菌酵母新型隐球菌是一种重要的人类病原体。它主要感染免疫功能低下的个体，引发脑膜脑炎，若不治疗则会致命。其遗传学和分子生物学的最新进展使其成为理解担子菌门和真菌致病机制的模型系统。实验操作相对简便，加上人类疾病小鼠模型的发展，使得对毒力机制和宿主反应的深入研究成为可能。本章介绍了该生物体及其生命周期，然后提供了详细的分步方案，涵盖培养、基因组操作、核酸和蛋白质分析，以及毒力评估和毒力因子表达。
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