Glycotechnology Business Unit, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan.
Carbohydr Res. 2010 Nov 22;345(17):2485-90. doi: 10.1016/j.carres.2010.09.022. Epub 2010 Sep 25.
We confirmed that a recombinant α-(2→3)-sialyltransferase cloned from Photobacterium sp. JT-ISH-224 recognizes inositols having a structure corresponding to the C-3 and C-4 of a galactopyranoside moiety, such as epi-, 1d-chiro, myo-, and muco-inositol, as acceptor substrates, and that the enzyme can transfer N-acetylneuraminic acid (Neu5Ac) from cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) to them. After purifying the reaction products, the structures were confirmed by use of NMR spectroscopy and mass spectrometry. From these results, it was clearly shown that the α-(2→3)-sialyltransferase from Photobacterium sp. JT-ISH-224 recognizes acceptor substrates through the cis-diol structure corresponding to the 3- and 4-position of the galactopyranoside moiety.
我们证实，从 Photobacterium sp. JT-ISH-224 克隆的重组 α-(2→3)-唾液酸转移酶识别具有半乳糖吡喃糖苷部分的 C-3 和 C-4 结构的肌醇，如表肌醇、1d-差向肌醇、肌醇和粘肌醇，作为受体底物，并且该酶可以将 N-乙酰神经氨酸（Neu5Ac）从胞苷 5'-单磷酸-N-乙酰神经氨酸（CMP-Neu5Ac）转移到它们上。在纯化反应产物后，通过使用 NMR 光谱和质谱法确认了结构。从这些结果清楚地表明，来自 Photobacterium sp. 的 α-(2→3)-唾液酸转移酶 JT-ISH-224 通过半乳糖吡喃糖苷部分的 3-和 4-位对应的顺二醇结构识别受体底物。
