提高酿酒酵母半乳糖诱导系统的性能。
Improvement of galactose induction system in Saccharomyces cerevisiae.
机构信息
Matsuyama Research Group, Toyota central R&D Labs. Inc., 41-1 Nagakute-yokomichi, Nagakute-cho, Aichi 480-1192, Japan.
出版信息
J Biosci Bioeng. 2011 Feb;111(2):175-7. doi: 10.1016/j.jbiosc.2010.09.014. Epub 2010 Oct 13.
PMID:20947423
Abstract
Here we report a significant enhancement of galactose response without altering the characteristics of glucose repression. To improve the galactose response, we fabricated transgenic yeasts harboring HIS3pro-GAL1, HIS3pro-GAL2 and GAL10pro-GAL4, and evaluated the synergistic effects of these three genes by immunoblot and flow cytometry analyses.
摘要
在这里，我们报道了在不改变葡萄糖抑制特性的情况下，显著增强了半乳糖响应。为了改善半乳糖响应，我们构建了携带 HIS3pro-GAL1、HIS3pro-GAL2 和 GAL10pro-GAL4 的转基因酵母，并通过免疫印迹和流式细胞术分析评估了这三个基因的协同效应。
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