Lucassen R, Schulte-Pelkum J, Csuvarszki C, Kleine-Tebbe J, Fooke M, Mahler M
Dr. Fooke Laboratorien GmbH, Mainstraße 85, 41469 Neuss, Germany.
J Allergy (Cairo). 2010;2010. doi: 10.1155/2010/524084. Epub 2010 Jun 6.
Type I hypersensitivity is driven by allergen specific immunoglobulin E (sIgE) and thus sIgE represents a marker for modern allergy diagnosis. Recently, a rapid assay for the detection of sIgE, termed as (Allergy Lateral Flow Assay) ALFA, has been developed. The objective of our study is the evaluation of a scanner-based system for the semiquantitative interpretation of ALFA results. Agreement to Skin Prick Test (SPT, Allergopharma), ALLERG-O-LIQ System (Dr. Fooke), and ImmunoCAP (Phadia) was investigated using 50 sera tested for specific IgE to timothy grass pollen (g6). 35/50 sera were positive by SPT, ALLERG-O-LIQ, and ImmunoCAP. Excellent agreement was observed between ALFA results and SPT, ImmunoCAP, and ALLERG-O-LIQ. Area under the curve (AUC) values were found at 1.0, and 100% sensitivity and specificity was found versus all other methods. Visual- and scanner-based interpretation of the ALFA results revealed excellent agreement.
I型超敏反应由变应原特异性免疫球蛋白E（sIgE）驱动，因此sIgE是现代过敏诊断的一个标志物。最近，一种用于检测sIgE的快速检测方法，称为过敏侧向流动分析（ALFA），已经被开发出来。我们研究的目的是评估一种基于扫描仪的系统，用于对ALFA结果进行半定量解释。使用50份针对梯牧草花粉（g6）特异性IgE进行检测的血清，研究了其与皮肤点刺试验（SPT，Allergopharma）、ALLERG-O-LIQ系统（Dr. Fooke）和ImmunoCAP（Phadia）的一致性。50份血清中有35份通过SPT、ALLERG-O-LIQ和ImmunoCAP检测为阳性。在ALFA结果与SPT、ImmunoCAP和ALLERG-O-LIQ之间观察到极好的一致性。曲线下面积（AUC）值为1.0，与所有其他方法相比，敏感性和特异性均为100%。基于视觉和扫描仪的ALFA结果解释显示出极好的一致性。
