Allergy Clinic, Copenhagen University Hospital, Gentofte, Denmark.
Clin Transl Allergy. 2011 Dec 9;1(1):15. doi: 10.1186/2045-7022-1-15.
The botanical relation between grass and cereal grains may be relevant when diagnosing food allergy to cereals. The aim was to investigate the diagnostic specificity of skin prick test (SPT) and specific immunoglobulin E (sIgE) tests to cereals and peanut in grass pollen allergic subjects without history of, and clinically reactions to foods botanically related to grass.
70 subjects (41 females; mean age 32 years) and 20 healthy controls (13 females; mean age 24 years) were tested by open food challenge (OFC) with cereals and peanut. SPT and sIgE both with Immulite® (Siemens) and ImmunoCAP® (Phadia) to grass and birch pollen, cereals, peanut and bromelain were performed.
Of the 65 OFC-negative subjects 29-46% (SPT, depending on cut-off), 20% (Immulite) and 38% (ImmunoCAP) had positive results to one or more of the foods tested. Controls were negative in all tests. Cross-reactive carbohydrate determinants (CCD) as evidenced by reaction to bromelain could explain only a minority of the measured IgE-sensitizations.
Grass pollen allergic patients with documented food tolerance to cereals and peanut may express significant sensitization. False-positive cereal or peanut allergy diagnoses may be a quantitatively important problem both in routine clinical work and epidemiological studies.
在诊断对谷物的食物过敏时，草类和谷物之间的植物学关系可能具有相关性。目的是研究在没有草类相关食物过敏史和临床过敏反应的情况下，对草花粉过敏的受试者进行皮肤点刺试验（SPT）和特定免疫球蛋白 E（sIgE）检测对谷物和花生的诊断特异性。
70 名受试者（41 名女性；平均年龄 32 岁）和 20 名健康对照者（13 名女性；平均年龄 24 岁）接受了谷物和花生的开放食物挑战（OFC）。使用 Immulite®（西门子）和 ImmunoCAP®（Phadia）进行草类和桦树花粉、谷物、花生和菠萝蛋白酶的 SPT 和 sIgE 检测。
在 65 名 OFC 阴性的受试者中，29-46%（SPT，取决于截断值）、20%（Immulite）和 38%（ImmunoCAP）对一种或多种测试的食物呈阳性反应。对照者在所有检测中均为阴性。交叉反应性碳水化合物决定簇（CCD），如对菠萝蛋白酶的反应，可以解释仅少数测量的 IgE 致敏情况。
有记录的谷物和花生食物耐受的草花粉过敏患者可能表现出显著的致敏。在常规临床工作和流行病学研究中，对谷物或花生过敏的假阳性诊断可能是一个数量上重要的问题。
