Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil.
PLoS One. 2010 Oct 8;5(10):e13205. doi: 10.1371/journal.pone.0013205.
While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia.
METHODOLOGY/PRINCIPAL FINDINGS: A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue.
CONCLUSIONS/SIGNIFICANCE: This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.
虽然 microRNAs（miRNAs）在组织分化和维持基础生理功能方面发挥着重要作用，但人们对胃组织中的 miRNA 表达水平知之甚少。miRNA 谱的改变会导致细胞失调，从而诱发肿瘤。
方法/主要发现：使用高通量 SOLiD 测序技术对胃组织的小 RNA 文库进行测序。我们获得了 261274 条与人类 miRNAome 完全匹配的高质量读数，其中 42%的已知 miRNA 被鉴定出来。基于读数丰度进行数字基因表达谱（DGE）分析，结果表明 15 种 miRNAs 在胃组织中高度表达。随后，通过 RT-PCR 在 10 名健康个体中验证了这些 miRNA 的表达，结果显示其表达的相关性为 83.97%（P<0.05）。6 种 miRNA 表现出低变异性表达模式（miR-29b、miR-29c、miR-19b、miR-31、miR-148a、miR-451），可被视为健康胃组织表达模式的一部分。
结论/意义：本研究旨在验证人胃组织正常的 miRNA 谱，建立健康个体的参考图谱。确定在胃中起作用的调控过程对于对抗全球第二大癌症死亡原因——胃癌至关重要。
