Key Laboratory of Tropical Marine Environment Dynamics (LED), South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou 510301, China.
Appl Microbiol Biotechnol. 2011 Feb;89(4):1233-41. doi: 10.1007/s00253-010-2925-4. Epub 2010 Oct 15.
The community structure and diversity of anaerobic ammonium oxidation (anammox) bacteria in the surface sediments of equatorial Pacific were investigated by phylogenic analysis of 16S rRNA and hydrazine oxidoreductase (hzo) genes and PCoA (principal coordinates analysis) statistical analysis. Results indicated that 16S rRNA and hzo sequences in the P2 (off the center of western Pacific warm pool) and P3 (in the eastern equatorial Pacific) sites all belong to the Candidatus "Scalindua", the dominate anammox bacteria in the low-temperature marine environment proved by previous studies. However, in the P1 site (in center of warm pool of western Pacific), large part of 16S rRNA gene sequences formed a separated cluster. Meanwhile, hzo gene sequences from P1 sediment also grouped into a single cluster. PCoA analysis demonstrated that the anammox community structure in the P1 has significant geographical distributional difference from that of P2, P3, and other marine environments based on 16S rRNA and hzo genes. The abundances of anammox bacteria in surface sediments of equatorial Pacific were quantified by q-PCR analysis of hzo genes, which ranged from 3.98 × 10(3) to 1.17 × 10(4) copies g(-1) dry sediments. These results suggested that a special anammox bacteria phylotypes exist in the surface sediment of the western Pacific warm pool, which adapted to the specific habitat and maybe involved in the nitrogen loss process from the fixed inventory in the habitat.
通过对赤道太平洋表层沉积物中厌氧氨氧化（anammox）细菌的 16S rRNA 和肼氧化还原酶（hzo）基因进行系统发育分析和 PCoA（主坐标分析）统计分析，研究了其群落结构和多样性。结果表明，P2（西太平洋暖池中心以外）和 P3（东赤道太平洋）站位的 16S rRNA 和 hzo 序列均属于先前研究证明的低温海洋环境中占优势的厌氧氨氧化菌“Scalindua”。然而，在 P1 站位（西太平洋暖池中心），大部分 16S rRNA 基因序列形成了一个单独的聚类。同时，P1 沉积物中的 hzo 基因序列也聚成一个单独的聚类。PCoA 分析表明，基于 16S rRNA 和 hzo 基因，P1 站位的厌氧氨氧化群落结构与 P2、P3 站位及其他海洋环境有显著的地理分布差异。通过 hzo 基因的 q-PCR 分析定量了赤道太平洋表层沉积物中厌氧氨氧化菌的丰度，范围为 3.98×10(3)~1.17×10(4)拷贝 g(-1)干沉积物。这些结果表明，西太平洋暖池表层沉积物中存在一种特殊的厌氧氨氧化菌，它们适应特定的生境，并可能参与该生境中固定氮库的氮损失过程。
