Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan.
Biomed Microdevices. 2011 Feb;13(1):117-22. doi: 10.1007/s10544-010-9476-4.
When nerve growth factor (NGF) is interacted with PC12 cells derived from rat pheochromocytoma, they are partially differentiated into neuron-like cells with neurites. In this work, PC12 cells differentiated by NGF were selectively isolated using a localized impulsive force in a μm-scale area, which was generated by focusing an infrared femtosecond laser into a cell culture medium. In order to evaluate the ability of the isolation method, differentiated and undifferentiated cells were isolated and their morphological changes after the isolation were compared. In both cases, their neurites were once contracted and some of them gradually regenerated day by day. When differentiated cells were isolated, the percentage of differentiated cells with regenerated neurites, 6 h after the isolation, was about 3.3 times higher than that when undifferentiated ones were isolated. This result was compared with a control trypsin experiment. In the comparison, it was indicated that the same degree of cell function was maintained when the present isolation method was used.
当神经生长因子（NGF）与源自大鼠嗜铬细胞瘤的 PC12 细胞相互作用时，它们会部分分化为具有神经突的神经元样细胞。在这项工作中，使用聚焦在细胞培养基中的红外飞秒激光在μm 范围内产生的局部脉冲力，选择性地分离了由 NGF 分化的 PC12 细胞。为了评估分离方法的能力，分离了分化和未分化的细胞，并比较了它们在分离后的形态变化。在这两种情况下，它们的神经突都曾收缩，其中一些在一天天地逐渐再生。当分离分化细胞时，分离后 6 小时再生神经突的分化细胞的百分比约为未分化细胞的 3.3 倍。将该结果与对照胰蛋白酶实验进行了比较。在比较中，当使用本发明的分离方法时，表明保持了相同程度的细胞功能。
