评价油剂绿僵菌与低剂量吡丙醚复配防治水稻褐飞虱的增效作用。

Evaluation of alternative rice planthopper control by the combined action of oil-formulated Metarhizium anisopliae and low-rate buprofezin.

机构信息

Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

出版信息

Pest Manag Sci. 2011 Jan;67(1):36-43. doi: 10.1002/ps.2026. Epub 2010 Oct 14.

DOI:10.1002/ps.2026

PMID:20949549

Abstract

BACKGROUND

High resistance of brown planthopper (BPH) Nilaparvata lugens Stål to common insecticides is a challenge for control of the pest. An alternative control strategy based on the combined application of fungal and chemical agents has been evaluated.

RESULTS

Three gradient spore concentrations of oil-formulated Metarhizium anisopliae (Metschnikoff) Sorokin (Ma456) were sprayed onto third-instar nymphs in five bioassays comprising the low buprofezin rates of 0, 10, 20, 30 and 40 µg mL(-1) respectively. Fungal LC(50) after 1 week at 25 °C and 14:10 h light:dark photoperiod decreased from 386 conidia mm(-2) in the buprofezin-free bioassay to 40 at the highest chemical rate. Buprofezin (LC(50): 1647, 486 and 233 µg mL(-1) on days 2 to 4) had no significant effect on the fungal outgrowths of mycosis-killed cadavers at the low application rates. The fungal infection was found to cause 81% reduction in reproductive potential of BPH adults. In two 40 day field trials, significant planthopper (mainly BPH) control (54-60%) was achieved by biweekly sprays of two fungal candidates (Ma456 and Ma576) at 1.5 × 10(13) conidia ha(-1) and elevated to 80-83% by incorporating 30.8 g buprofezin ha(-1) into the fungal sprays.

CONCLUSION

The combined application of the fungal and chemical agents is a promising alternative strategy for BPH control.

摘要

背景

褐飞虱（BPH）Nilaparvata lugens Stål 对常用杀虫剂的高抗性是该害虫防治的一个挑战。已评估了一种基于真菌和化学药剂联合应用的替代控制策略。

结果

在包含低吡虫啉浓度 0、10、20、30 和 40μg mL(-1) 的五个生物测定中，将油剂型玫烟色棒束孢（Metschnikoff）Sorokin（Ma456）的三个梯度孢子浓度分别喷洒到三龄若虫上。在 25°C 和 14:10 h 光照：黑暗光周期下，1 周后真菌 LC(50)从无吡虫啉生物测定中的 386 个分生孢子 mm(-2)下降到最高化学浓度下的 40 个。吡虫啉（LC(50)：在第 2 至 4 天分别为 1647、486 和 233μg mL(-1)）对低剂量应用时真菌致死尸体的真菌生长没有显著影响。发现真菌感染导致 BPH 成虫生殖能力下降 81%。在两个为期 40 天的田间试验中，通过每两周喷洒两次真菌候选物（Ma456 和 Ma576），浓度为 1.5×10(13)个分生孢子 ha(-1)，并将 30.8 g 吡虫啉 ha(-1) 加入真菌喷雾中，可实现显著的褐飞虱（主要是 BPH）防治效果（54-60%）。