Wang Yanhua, Gao Congfen, Xu Zhiping, Zhu Yu Cheng, Zhang Jiushuang, Li Wenhong, Dai Dejiang, Lin Youwei, Zhou Weijun, Shen Jinliang
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture/Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Pest Manag Sci. 2008 Oct;64(10):1050-6. doi: 10.1002/ps.1606.
Buprofezin has been used for many years to control Nilaparvata lugens (Stål). Assessment of susceptibility change in the insect is essential for maintaining control efficiency and resistance management.
Eleven-year surveys showed that most field populations were susceptible before 2004. However, substantially higher levels of resistance (up to 28-fold) were found in most of the rice fields in China after 2004. A field population was collected and periodically selected for buprofezin resistance in the laboratory. After 65 generations (56 were selected), the colony successfully obtained 3599-fold resistance to buprofezin. Synergism tests showed that O,O-diethyl-O-phenyl phosphorothioate (SV1), piperonyl butoxide (PBO) and diethyl maleate (DEM) increased buprofezin toxicity in the resistant strain by only 1.5-1.6 fold, suggesting that esterases, P450-monooxygenases and glutathione S-transferases had no substantial effect on buprofezin resistance development.
The results from this study indicate that N. lugens has the potential to develop high resistance to buprofezin. A resistance management program with rotation of buprofezin and other pesticides may efficiently delay or slow down resistance development in the insect. Further investigation is also necessary to understand the resistance mechanisms in N. lugens.
噻嗪酮已被用于防治褐飞虱多年。评估该昆虫的敏感性变化对于维持防治效果和抗性治理至关重要。
为期11年的调查显示，2004年之前大多数田间种群对噻嗪酮敏感。然而，2004年之后在中国的大多数稻田中发现了抗性水平大幅提高（高达28倍）的情况。采集了一个田间种群并在实验室中定期进行噻嗪酮抗性选育。经过65代（其中56代进行了选育）后，该群体成功获得了对噻嗪酮3599倍的抗性。增效试验表明，O,O-二乙基-O-苯基硫代磷酸酯（SV1）、胡椒基丁醚（PBO）和顺丁烯二酸二乙酯（DEM）仅使抗性品系中噻嗪酮的毒性提高了1.5 - 1.6倍，这表明酯酶、P450单加氧酶和谷胱甘肽S-转移酶对噻嗪酮抗性的发展没有实质性影响。
本研究结果表明褐飞虱具有对噻嗪酮产生高抗性的潜力。采用噻嗪酮与其他杀虫剂轮换使用的抗性治理方案可能有效地延缓或减缓该昆虫抗性的发展。还需要进一步研究以了解褐飞虱的抗性机制。
