Trapani J A, Dupont B
Department of Pathology, University of Melbourne, Parkville, Australia.
Tissue Antigens. 1990 Nov;36(5):228-34. doi: 10.1111/j.1399-0039.1990.tb01833.x.
This report describes the organization of the mouse pore-forming protein (perforin) gene (Pfp), which is highly analogous to that of the corresponding human gene. Pfp comprises three exons, the first of which consists entirely of 5' non-coding sequence, separated by an intron of 1.94 kb from the two polypeptide-coding exons. The promoter region of the gene shows strong similarity to that in humans, with six stretches of high homology noted within 0.7 kb of the mRNA cap site. However, many of the sequences of the human gene with similarity to previously described promoter/enhancer elements are poorly conserved in the mouse, suggesting that these motifs may be of no functional significance, and that control mechanisms for expression of Pfp may be highly specific to killer cells.
本报告描述了小鼠穿孔素基因（Pfp）的结构组织，该基因与相应的人类基因高度相似。Pfp由三个外显子组成，其中第一个外显子完全由5'非编码序列构成，与另外两个编码多肽的外显子之间被一个1.94 kb的内含子隔开。该基因的启动子区域与人类基因的启动子区域有很强的相似性，在mRNA帽位点的0.7 kb范围内有六个高度同源的片段。然而，人类基因中许多与先前描述的启动子/增强子元件相似的序列在小鼠中保守性较差，这表明这些基序可能没有功能意义，并且Pfp表达的调控机制可能对杀伤细胞具有高度特异性。
