Trapani J A, Kwon B S, Kozak C A, Chintamaneni C, Young J D, Dupont B
Laboratory of Human Immunogenetics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
J Exp Med. 1990 Feb 1;171(2):545-57. doi: 10.1084/jem.171.2.545.
Genomic clones encompassing the entire coding region of the mouse lymphocyte pore-forming protein gene (Pfp) have been isolated and used to determine its intron-exon organization. In contrast to C9, Pfp has a simple structure, consisting of only three exons (two of which encode polypeptide), a large 5' intron, and a single, smaller intron that is situated approximately one-third of the way through the protein-coding portions of the gene. The regions encoding the homologous domains of PFP and C9 are encoded on exons 7, 8, 9, and 10 of C9, but form only approximately half of the open reading frame of exon III in Pfp. Although encoding polypeptides with related functions, the two genes possess such sharply contrasting structures as to suggest that their analogous regions may have risen independently, by a process of convergent evolution. Using a panel of somatic cell hybrid cell lines, Pfp has been mapped to chromosome 10.
包含小鼠淋巴细胞成孔蛋白基因（Pfp）整个编码区的基因组克隆已被分离出来，并用于确定其内含子-外显子结构。与C9不同，Pfp结构简单，仅由三个外显子（其中两个编码多肽）、一个大的5'内含子和一个较小的单一内含子组成，该内含子位于基因蛋白质编码部分大约三分之一的位置。编码PFP和C9同源结构域的区域位于C9的外显子7、8、9和10上，但在Pfp中仅构成外显子III开放阅读框的大约一半。尽管这两个基因编码具有相关功能的多肽，但它们的结构形成了鲜明对比，这表明它们的类似区域可能是通过趋同进化过程独立产生的。利用一组体细胞杂交细胞系，已将Pfp定位到10号染色体上。
