Rønningen K S, Spurkland A, Egeland T, Iwe T, Munthe E, Vartdal F, Thorsby E
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Tissue Antigens. 1990 Nov;36(5):235-40. doi: 10.1111/j.1399-0039.1990.tb01834.x.
Genomic typing of in vitro amplified DNA with sequence-specific oligonucleotide (SSO) probes was performed for DRB1, DQA1, DQB1, DPA1 and DPB1 alleles in 54 random Norwegian rheumatoid arthritis (RA) patients and 181 healthy controls. DRB1 alleles encoding the serological specificity DR4 were found in 80% of the patients, compared to 34% of the controls (relative risk = 7.9, p less than 0.0001). All DR4-positive RA patients carried either DRB10401 (Dw4), 0404 (Dw14), or 0405 (Dw15), while no patients were found to carry DRB10402 (Dw10) or 0403 (Dw13). The frequency of the DRB1*0101 allele encoding DR1 was not increased, even among DR4-negative RA patients, and we were unable to detect any sharing of other class II alleles among DR4-negative patients. No contribution of any DQA1, DQB1, DPA1 or DPB1 alleles to RA susceptibility could be detected. The results suggest that in the Norwegian population RA is primarily associated with a shared sequence at residues 67-74 of the DR beta 1 chain, but only when this sequence is expressed on DR4 molecules.
运用序列特异性寡核苷酸（SSO）探针，对54例随机选取的挪威类风湿关节炎（RA）患者及181名健康对照者的DRB1、DQA1、DQB1、DPA1和DPB1等位基因进行体外扩增DNA的基因分型。在80%的患者中发现了编码血清学特异性DR4的DRB1等位基因，而在对照者中这一比例为34%（相对风险=7.9，p<0.0001）。所有DR4阳性的RA患者携带的均为DRB10401（Dw4）、0404（Dw14）或0405（Dw15），未发现携带DRB10402（Dw10）或0403（Dw13）的患者。编码DR1的DRB1*0101等位基因频率并未升高，即便在DR4阴性的RA患者中也是如此，并且我们未能在DR4阴性患者中检测到其他II类等位基因的共享情况。未检测到任何DQA1、DQB1、DPA1或DPB1等位基因对RA易感性有贡献。结果表明，在挪威人群中，RA主要与DRβ1链67 - 74位残基处的一个共享序列相关，但仅当该序列在DR4分子上表达时才会如此。
