含 N-乙酰半胱氨酸的牙本质粘结剂的体外细胞相容性。

In vitro cytocompatibility of N-acetylcysteine-supplemented dentin bonding agents.

机构信息

Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.

出版信息

J Endod. 2010 Nov;36(11):1844-50. doi: 10.1016/j.joen.2010.08.005. Epub 2010 Sep 16.

DOI:10.1016/j.joen.2010.08.005

PMID:20951298

Abstract

INTRODUCTION

Cytotoxic resin components of dentin bonding agents are known to cause oxidative damage and suppress odontogenic differentiation of dental pulp cells. Because antioxidants were found to protect cells from cytotoxicity of resin monomers in previous studies, we investigated the effect of N-acetylcysteine (NAC) on cytotoxicity and anti-differentiation activity of bonding agents.

METHODS

Human dental pulp cells were treated with the extracts of dentin bonding agents (Prime & Bond NT, Adper Single Bond, and Dentin Cement), and then cell viability, alkaline phosphatase (ALP) activity, and matrix mineralization were observed. To assess the effects of NAC, NAC was directly added into culture media or mixed with bonding agents before extraction. Release of NAC from bonding agents was also observed by high-performance liquid chromatography.

RESULTS

NAC enhanced ALP activity and mRNA expression of dentin sialophosphoprotein gene, whereas extracts of dentin bonding agents inhibited the induction of ALP activity. When the cells were treated with extracts of the bonding agents, the NAC in the culture media reduced the cytotoxicity of the bonding agents. When NAC was incorporated into bonding agents, a protective effect was only seen for Prime & Bond NT containing more than 1% NAC. The disruption of ALP activity and matrix mineralization in pulp cells was partially reversed by NAC only in Prime & Bond NT-treated cells. High-performance liquid chromatography analysis of NAC showed that the amount of NAC effluxed from Prime & Bond NT was not greater than that effluxed from Adper Single Bond.

CONCLUSIONS

NAC was useful for reversing cytotoxicity and anti-differentiation effects of Prime & Bond NT on human dental pulp cells.

摘要

简介

牙本质粘结剂中的细胞毒性树脂成分已知会引起氧化损伤，并抑制牙髓细胞的牙源性分化。由于抗氧化剂在前瞻性研究中被发现可以保护细胞免受树脂单体的细胞毒性作用，因此我们研究了 N-乙酰半胱氨酸（NAC）对粘结剂的细胞毒性和抗分化活性的影响。

方法

用人牙髓细胞处理牙本质粘结剂（Prime & Bond NT、Adper Single Bond 和 Dentin Cement）的提取物，然后观察细胞活力、碱性磷酸酶（ALP）活性和基质矿化。为了评估 NAC 的作用，将 NAC 直接添加到培养基中或在提取前与粘结剂混合。通过高效液相色谱法观察 NAC 从粘结剂中的释放情况。

结果

NAC 增强了 ALP 活性和牙本质涎磷蛋白基因的 mRNA 表达，而牙本质粘结剂的提取物抑制了 ALP 活性的诱导。当细胞用粘结剂的提取物处理时，培养基中的 NAC 降低了粘结剂的细胞毒性。当 NAC 被掺入粘结剂中时，只有 Prime & Bond NT 中含有超过 1%的 NAC 才有保护作用。只有在 Prime & Bond NT 处理的细胞中，NAC 部分逆转了 ALP 活性和基质矿化的破坏。高效液相色谱法分析 NAC 显示，从 Prime & Bond NT 中流出的 NAC 量不大于从 Adper Single Bond 中流出的 NAC 量。