Department of Restorative Dentistry, Ege University School of Dentistry, 35100, Izmir, Turkey.
Department of Restorative Dentistry, Selcuk University School of Dentistry, Konya, Turkey.
Clin Oral Investig. 2019 Aug;23(8):3213-3218. doi: 10.1007/s00784-018-2745-y. Epub 2018 Nov 10.
The aim of this in vitro study is to evaluate the effects of resveratrol (RES) addition on the cytotoxicity and microtensile bond strength (μTBS) of different adhesives.
Five self-etching adhesives (G-aenial Bond-GC, Optibond All in One-Kerr, Gluma Self Etch-Kulzer, Clearfil S Bond-Kuraray, and Nova Compo-B Plus-Imicryl) were tested. They were applied to L-929 cell culture by the extract method. In the test groups, 0.5 μM RES (Sigma-Aldrich) was added into the medium. Cell viability was assessed by MTT assay after 24 h. Human extracted third molars were used for μTBS test (n = 7). The adhesives with or without 0.5 μM RES addition were applied on dentin surfaces. A composite build-up was constructed. Then, the specimens were sectioned into multiple beams with the non-trimming version of the microtensile test and subjected to microtensile forces. Statistical analysis was performed using ANOVA and post hoc Tukey test (p ˂ 0.05).
The extracts of all adhesives decreased the cell viability. However, RES addition increased the cell viability in all groups (p ˂ 0.05). RES addition did not cause any decrease in μTBS values of the adhesives compared to baseline. Optibond All in One showed the highest μTBS after RES addition. It was followed by Clerafil S Bond and Nova Compo-B Plus. No difference was determined between the Optibond All in One and Clearfil S Bond. There was difference between Optibond All in One and Nova Compo-B Plus (p ˂ 0.05).
RES addition may improve the biocompatibility without causing negative influence on μTBS of the adhesives.
RES addition has clinical applicable potential to overcome the adverse biocompatibility of adhesives.
本体外研究旨在评估白藜芦醇（RES）添加对不同胶粘剂细胞毒性和微拉伸结合强度（μTBS）的影响。
测试了 5 种自酸蚀胶粘剂（G-aenial Bond-GC、Optibond All in One-Kerr、Gluma Self Etch-Kulzer、Clearfil S Bond-Kuraray 和 Nova Compo-B Plus-Imicryl）。采用浸提法将其应用于 L-929 细胞培养。在实验组中，向培养基中加入 0.5μM 的 RES（Sigma-Aldrich）。24 小时后通过 MTT 测定法评估细胞活力。采用人第三磨牙提取标本进行 μTBS 测试（n=7）。将含有或不含有 0.5μM RES 添加物的胶粘剂应用于牙本质表面。构建复合层。然后，将试件用微拉伸试验的非修剪版本切成多个梁，并施加微拉伸力。使用方差分析和事后 Tukey 检验（p<0.05）进行统计分析。
所有胶粘剂的提取物均降低了细胞活力。然而，RES 添加剂在所有组中均增加了细胞活力（p<0.05）。与基线相比，RES 添加物未导致胶粘剂 μTBS 值降低。添加 RES 后，Optibond All in One 的 μTBS 最高。其次是 Clearfil S Bond 和 Nova Compo-B Plus。Optibond All in One 和 Clearfil S Bond 之间没有差异。Optibond All in One 和 Nova Compo-B Plus 之间存在差异（p<0.05）。
RES 添加物可能会改善生物相容性，而不会对胶粘剂的 μTBS 产生负面影响。
RES 添加物具有潜在的临床应用价值，可以克服胶粘剂的不良反应性。
