Institut de Recherches en Technologie et Sciences pour le Vivant IRTSV-LCBM, UMR 5249 CEA/CNRS/UJF, CEA-Grenoble, 17 avenue des Martyrs, 38054 Grenoble Cedex 09, France.
Curr Opin Struct Biol. 2010 Dec;20(6):684-92. doi: 10.1016/j.sbi.2010.09.009. Epub 2010 Oct 14.
Proteins and RNA molecules enjoy a variety of chemically complex post-translational and post-transcriptional modifications. The chemistry at work in these reactions, which was considered to be exclusively ionic in nature has recently been shown to depend on radical mechanisms in some cases. The overwhelming majority of these radical-based reactions are catalyzed by 'Radical-SAM' enzymes. This review article highlights mechanistic and structural aspects of this class of reactions and indicates important research directions to be addressed.
蛋白质和 RNA 分子享有各种化学复杂的翻译后和转录后修饰。这些反应中的化学作用，过去被认为仅具有离子性质，最近在某些情况下已显示依赖于自由基机制。这些基于自由基的反应绝大多数由“Radical-SAM”酶催化。本文综述了该类反应的机制和结构方面，并指出了需要解决的重要研究方向。
