由自由基-SAM酶介导的甲硫基化反应。
The methylthiolation reaction mediated by the Radical-SAM enzymes.
作者信息
Atta Mohamed, Arragain Simon, Fontecave Marc, Mulliez Etienne, Hunt John F, Luff Jon D, Forouhar Farhad
机构信息
Institut de Recherches en Technologie et Sciences pour le Vivant IRTSV-LCBM, UMR 5249 CEA/CNRS/UJF, CEA-Grenoble, 17 avenue des Martyrs, 38054, Grenoble Cedex 09, France.
出版信息
Biochim Biophys Acta. 2012 Nov;1824(11):1223-30. doi: 10.1016/j.bbapap.2011.11.007. Epub 2011 Dec 7.
Abstract
Over the past 10 years, considerable progress has been made in our understanding of the mechanistic enzymology of the Radical-SAM enzymes. It is now clear that these enzymes appear to be involved in a remarkably wide range of chemically challenging reactions. This review article highlights mechanistic and structural aspects of the methylthiotransferases (MTTases) sub-class of the Radical-SAM enzymes. The mechanism of methylthio insertion, now observed to be performed by three different enzymes is an exciting unsolved problem. This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology.
摘要
在过去十年中，我们对自由基S-腺苷甲硫氨酸（Radical-SAM）酶的机制酶学有了相当大的进展。现在很清楚，这些酶似乎参与了范围极为广泛的具有化学挑战性的反应。这篇综述文章重点介绍了Radical-SAM酶亚类甲基硫转移酶（MTTases）的机制和结构方面。目前观察到由三种不同酶进行的甲硫基插入机制是一个令人兴奋的未解决问题。本文是名为“自由基SAM酶与自由基酶学”特刊的一部分。
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