携带II型限制性核酸内切酶的天蓝色链霉菌菌株对噬菌体的敏感性。
Sensitivity to phages of Streptomyces coelicolor strains harbouring type II restriction endonucleases.
作者信息
Aparicio J F, Barbés C, Hardisson C, Sánchez J
机构信息
Department of Functional Biology, University of Oviedo, Spain.
出版信息
Microbiologia. 1990 Dec;6(2):71-5.
The role of type II restriction endonucleases in phage development in two different strains of Streptomyces coelicolor has been analyzed. Two of ten phages tested (phi A4 R4c 1) presented a low efficiency of plating (e.o.p.) in the studied strains. The isolation of host-range mutants of phi A4 and R4c 1, with improved e.o.p. and higher adsorption capability in these two bacterial strains, suggests that the presence of host endonucleases is not the main barrier for these phages, but rather adsorption inability.
已经分析了II型限制性核酸内切酶在两种不同天蓝色链霉菌菌株噬菌体发育中的作用。在所测试的10种噬菌体中有两种（phi A4 R4c 1）在研究的菌株中呈现出低平板效率（e.o.p.）。分离出phi A4和R4c 1的宿主范围突变体，它们在这两种细菌菌株中的e.o.p.有所提高且吸附能力更强，这表明宿主核酸内切酶的存在不是这些噬菌体的主要障碍，而是吸附能力不足。
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