Institute of Evolutionary Biochemistry and Physiology RAS, pr Torez 44, Saint-Petersburg 194223, Russian Federation.
Fish Shellfish Immunol. 2011 Jan;30(1):434-8. doi: 10.1016/j.fsi.2010.10.002. Epub 2010 Oct 15.
In molluscs haemolymph lectins bearing fibrinogen-like domain (FREP) act as immune pattern-recognition receptors. A full-length cDNAs of MytFREP1 and MytFREP2 cloned from haemocytes of blue mussel Mytilus edulis encoded putative polypeptides of 230 and 241 amino acids. Both polypeptides consist of signal peptide and C-terminal fibrinogen-like domain. Immune functions of these molecules may be extrapolated from the close-related and functionally characterized lectin AiFREP from bay scallop, Argopecten irradians. However, immune challenge experiments with zymosan particles, Escherichia coli bacterium and cercariae of Himasthla elongata (Trematoda) failed to modulate MytFREP1 and MytFREP2 mRNA expression in M. edulis haemocytes. Hypothetically, it argues into rather high specificity of mechanisms triggering a differential expression of MytFREP genes. The search in the EST database revealed orthologous copies for described genes and portion of relatively similar genes from two close-related mytilids, Mytilus galloprovincialis and Mytilus californianus. We document the new multigene family of FREPs from bivalves of genus Mytilus. MytFREP family currently represented by 2 genes from M. edulis, 4 genes from M. californianus and 7 genes from M. galloprovincialis.
在软体动物的血淋巴中，具有纤维蛋白原样结构域（FREP）的凝集素作为免疫模式识别受体发挥作用。从贻贝 Mytilus edulis 的血细胞中克隆出全长 cDNA MytFREP1 和 MytFREP2，它们分别编码 230 和 241 个氨基酸的假定多肽。这两种多肽都由信号肽和 C 末端纤维蛋白原样结构域组成。这些分子的免疫功能可以从与之密切相关且具有功能特征的海湾扇贝凝集素 AiFREP 推断出来。然而，用几丁质颗粒、大肠杆菌细菌和海盘车尾索幼虫（扁形动物门）对贻贝血细胞进行免疫挑战实验，未能调节 MytFREP1 和 MytFREP2 的 mRNA 表达。从理论上讲，这表明触发 MytFREP 基因差异表达的机制具有相当高的特异性。在 EST 数据库中的搜索揭示了描述基因的同源拷贝以及来自两个密切相关的贻贝属，即加利福尼亚贻贝和马氏珠母贝的部分相对相似的基因。我们从贻贝属的双壳类动物中记录了新的 FREP 多基因家族。MytFREP 家族目前由来自 M. edulis 的 2 个基因、来自 M. californianus 的 4 个基因和来自 M. galloprovincialis 的 7 个基因组成。
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