Laboratory of Gastroenterology and Hepatology, São Paulo Institute of Tropical Medicine and Department of Gastroenterology, School of Medicine, University of São Paulo, Brazil.
Infect Genet Evol. 2011 Jan;11(1):103-8. doi: 10.1016/j.meegid.2010.10.003. Epub 2010 Oct 15.
Hepatitis B is a worldwide health problem affecting about 2 billion people and more than 350 million are chronic carriers of the virus. Nine HBV genotypes (A to I) have been described. The geographical distribution of HBV genotypes is not completely understood due to the limited number of samples from some parts of the world. One such example is Colombia, in which few studies have described the HBV genotypes. In this study, we characterized HBV genotypes in 143 HBsAg-positive volunteer blood donors from Colombia. A fragment of 1306 bp partially comprising HBsAg and the DNA polymerase coding regions (S/POL) was amplified and sequenced. Bayesian phylogenetic analyses were conducted using the Markov Chain Monte Carlo (MCMC) approach to obtain the maximum clade credibility (MCC) tree using BEAST v.1.5.3. Of all samples, 68 were positive and 52 were successfully sequenced. Genotype F was the most prevalent in this population (77%) - subgenotypes F3 (75%) and F1b (2%). Genotype G (7.7%) and subgenotype A2 (15.3%) were also found. Genotype G sequence analysis suggests distinct introductions of this genotype in the country. Furthermore, we estimated the time of the most recent common ancestor (TMRCA) for each HBV/F subgenotype and also for Colombian F3 sequences using two different datasets: (i) 77 sequences comprising 1306 bp of S/POL region and (ii) 283 sequences comprising 681 bp of S/POL region. We also used two other previously estimated evolutionary rates: (i) 2.60 × 10(-4)s/s/y and (ii) 1.5 × 10(-5)s/s/y. Here we report the HBV genotypes circulating in Colombia and estimated the TMRCA for the four different subgenotypes of genotype F.
乙型肝炎是一个全球性的健康问题，影响到约 20 亿人，其中超过 3.5 亿人为乙型肝炎病毒慢性携带者。已经描述了 9 种乙型肝炎病毒基因型（A 到 I）。由于世界上一些地区样本数量有限，乙型肝炎病毒基因型的地理分布尚不完全清楚。哥伦比亚就是一个例子，关于该国乙型肝炎病毒基因型的研究很少。在这项研究中，我们对来自哥伦比亚的 143 名 HBsAg 阳性志愿献血者的乙型肝炎病毒基因型进行了特征描述。扩增并测序了包含 HBsAg 和 DNA 聚合酶编码区（S/POL）的 1306bp 部分片段。使用贝叶斯系统发育分析（MCMC 方法）通过 BEAST v.1.5.3 获得最大分支可信度（MCC）树。在所有样本中，有 68 份为阳性，52 份成功测序。该人群中最常见的基因型是 F（77%）-亚基因型 F3（75%）和 F1b（2%）。还发现了基因型 G（7.7%）和亚基因型 A2（15.3%）。基因型 G 序列分析表明，该基因型在该国存在明显的传入。此外，我们使用两个不同的数据集，即（i）包含 S/POL 区域 1306bp 的 77 个序列和（ii）包含 S/POL 区域 681bp 的 283 个序列，对每个乙型肝炎病毒/F 亚基因型和哥伦比亚 F3 序列的最近共同祖先（TMRCA）进行了估计。我们还使用了另外两个先前估计的进化率：（i）2.60×10(-4)s/s/y 和（ii）1.5×10(-5)s/s/y。在这里，我们报告了在哥伦比亚流行的乙型肝炎病毒基因型，并估计了四个不同 F 基因型亚基因型的 TMRCA。
