Le Doan T, Praseuth D, Perrouault L, Chassignol M, Thuong N T, Hélène C
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U201, CNRS UA 481, Paris, France.
Bioconjug Chem. 1990 Mar-Apr;1(2):108-13. doi: 10.1021/bc00002a004.
Porphyrins linked to oligonucleotides produce various types of photodamage on a complementary target DNA. The observed reactions include oxidation of guanine bases and cross-linking reactions of the oligonucleotide to its target sequence. Guanines located close to the porphyrin macrocycle were the most altered as compared to more remote guanines on the target sequence. No specific reaction was observed when the complexes were dissociated at temperatures above the melting temperature of the oligonucleotide-target hybrid. Both cross-linking and oxidation reactions accounted for ca. 60% modification of the target chains in the complex. Our results show that oligonucleotides covalently linked to porphyrins are efficient systems for inducing irreversible sequence-specific photodamage on a target DNA.
与寡核苷酸相连的卟啉会对互补的靶标DNA产生多种类型的光损伤。观察到的反应包括鸟嘌呤碱基的氧化以及寡核苷酸与其靶标序列的交联反应。与靶标序列上距离较远的鸟嘌呤相比，靠近卟啉大环的鸟嘌呤变化最大。当复合物在高于寡核苷酸 - 靶标杂交体解链温度的温度下解离时，未观察到特异性反应。交联反应和氧化反应均约占复合物中靶标链修饰的60%。我们的结果表明，与卟啉共价相连的寡核苷酸是在靶标DNA上诱导不可逆序列特异性光损伤的有效体系。
