Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona 18, 08034 Barcelona, Spain.
Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona 18, 08034 Barcelona, Spain.
Environ Pollut. 2011 Jan;159(1):100-107. doi: 10.1016/j.envpol.2010.09.018. Epub 2010 Oct 16.
Development of stress markers for the invader freshwater zebra mussel (Dreissena polymorpha) is of great interest for both conservation and biomonitoring purposes. Gene expression profiles of several putative or already established gene expression stress markers (Metallothionein, Superoxide dismutase, Catalase, Glutathione S transferase, Glutathione peroxidase, Cytochrome c oxidase, the multixenobiotic resistance P-gp1, and heat shock proteins HSP70 and HSP90) were analyzed by quantitative Real-Time PCR in adults and pediveliger larvae after exposure to metals (Hg, Cu, Cd). A defined pattern of coordinated responses to metal exposure and, presumably, to oxidative stress was observed in gills and digestive gland from adults. A similar, albeit partial response was observed in larvae, indicating an early development of stress-related gene responses in zebra mussel. The tools developed in this study may be useful both for future control strategies and for the use of zebra mussel as sentinel species in water courses with stable populations.
开发入侵淡水贻贝（Dreissena polymorpha）的应激标志物对于保护和生物监测都具有重要意义。本研究通过定量实时 PCR 分析了几种潜在或已建立的基因表达应激标志物（金属硫蛋白、超氧化物歧化酶、过氧化氢酶、谷胱甘肽 S 转移酶、谷胱甘肽过氧化物酶、细胞色素 c 氧化酶、多药耐药 P-gp1 以及热休克蛋白 HSP70 和 HSP90）在成年贻贝和幼贝暴露于金属（Hg、Cu、Cd）后的基因表达谱。在成年贻贝的鳃和消化腺中观察到了对金属暴露和（可能）氧化应激的协调反应的明确模式。在幼虫中也观察到了类似的、尽管是部分的反应，表明斑马贻贝的应激相关基因反应在早期就已经发展。本研究中开发的工具对于未来的控制策略以及将斑马贻贝作为稳定种群的水道中的指示物种都可能具有重要意义。
