State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Bioresour Technol. 2011 Jan;102(2):1783-7. doi: 10.1016/j.biortech.2010.08.114. Epub 2010 Oct 16.
A novel fermentation strategy to enhance antibiotics production was demonstrated by inducing reactive oxygen species (ROS), and validamycin A (VAL-A) production by Streptomyces hygroscopicus 5008 in agro-industrial residues containing medium was taken as an example. By optimizing H2O2 amount and addition time, the intracellular ROS level was increased, and VAL-A production titer was enhanced by 40% on day 4 when 25 μM H2O2 was added at 8th h of fermentation. Addition of diphenyleneiodonium chloride (ROS inhibitor) reduced the H2O2 induction effect. The transcription level of eight VAL-A structure genes was enhanced by ROS, and activities of glucose-6-phosphate dehydrogenase and ValG enzyme were increased while glyceraldehyde 3-phosphate dehydrogenase activity was inhibited. This work demonstrated that ROS induction was a useful strategy for VAL-A fermentation, and the information on gene transcription and enzyme activities may be helpful to further understanding the mechanism of ROS effect on the antibiotic biosynthesis.
一种新型发酵策略通过诱导活性氧（ROS）来提高抗生素的产量，以吸水链霉菌 5008 在含有农业工业残留物的培养基中产生 validamycin A（VAL-A）为例。通过优化 H2O2 的用量和添加时间，增加了细胞内 ROS 水平，当在发酵 8 小时添加 25μM H2O2 时，第 4 天 VAL-A 的产量提高了 40%。添加二苯基碘氯化物（ROS 抑制剂）降低了 H2O2 的诱导作用。ROS 增强了八个 VAL-A 结构基因的转录水平，同时提高了葡萄糖-6-磷酸脱氢酶和 ValG 酶的活性，而甘油醛 3-磷酸脱氢酶的活性受到抑制。这项工作表明，ROS 诱导是 VAL-A 发酵的一种有效策略，有关基因转录和酶活性的信息可能有助于进一步了解 ROS 对抗生素生物合成的影响机制。
