State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, China.
Bioprocess Biosyst Eng. 2012 Oct;35(8):1309-16. doi: 10.1007/s00449-012-0718-0. Epub 2012 Apr 6.
In order to enhance the production of validamycin A (VAL-A), a widely used agricultural antibiotic, a temperature shift strategy was developed in the fermentation of Streptomyces hygroscopicus 5008. VAL-A production and the transcriptional levels of its structural genes were enhanced in the optimal temperature shift condition. The addition of diphenyleneiodonium [DPI, reactive oxygen species (ROS) inhibitor] inhibited intracellular ROS level and VAL-A production, which indicated that ROS signal might contribute to the enhancement of VAL-A production in the temperature shift process. The transcriptional levels of stress response sigma factors SigmaB and SigmaH as well as global regulator PhoRP were enhanced, which suggested that these regulators might participate in the signal pathway. This study developed a useful strategy for VAL-A production. It will help to further understand the regulation mechanism of ROS on VAL-A synthesis. The involvement of ROS in this process will encourage researchers to develop new ROS induction strategies to enhance VAL-A production.
为了提高有效霉素 A（VAL-A）的产量，一种广泛应用于农业的抗生素，我们在吸水链霉菌 5008 的发酵过程中开发了一种温度转换策略。在最佳温度转换条件下，VAL-A 的产量和其结构基因的转录水平都得到了提高。添加二苯基碘鎓 [DPI，活性氧（ROS）抑制剂] 抑制了细胞内 ROS 水平和 VAL-A 的产量，这表明 ROS 信号可能有助于在温度转换过程中提高 VAL-A 的产量。应激反应σ因子 SigmaB 和 SigmaH 以及全局调控因子 PhoRP 的转录水平都得到了增强，这表明这些调控因子可能参与了信号通路。本研究为 VAL-A 的生产开发了一种有用的策略。它将有助于进一步了解 ROS 对 VAL-A 合成的调控机制。ROS 在此过程中的参与将鼓励研究人员开发新的 ROS 诱导策略来提高 VAL-A 的产量。
