Wu Yugang, Jin Min, Xu Huanbai, Shimin Zhang, He Songbing, Wang Liang, Zhang Yanyun
Department of Surgery, The First People Hospital of Changzhou and The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Clin Dev Immunol. 2010;2010. doi: 10.1155/2010/537531. Epub 2010 Oct 7.
We investigated the clinicopathologic significance of HIF-1, CXCR4, and VEGF expression using immumohistochemistry in human colon cancer. HIF-1, CXCR4, and VEGF high expression levels were correlated positively with TNM stage, lymph node involvement, and distant metastasis Furthermore, we found that combined high expression of any two of the three molecules (P = .028 for HIF-1/CXCR4, P = .007 for HIF-1/VEGF, and P = .004 for CXCR4/VEGF) had stronger correlation with lymph node metastasis than did each alone. However, a relationship with distant metastasis is seen only with the combinations CXCR4/VEGF (P = .069 for HIF-1/CXCR4, P = .062 for HIF-1/VEGF, and P = .035 for CXCR4/VEGF) as compared with those of single molecule high expression alone. Combined expression of all three molecules strongly correlates with lymph node metastasis and distant metastasis. The mRNA expression of HIF-1, CXCR4, and VEGF were quantified by real-time PCR in different colon cancer tissue samples, the experiment results shown that fresh colon tissue samples significantly overexpressed CXCR4 and VEGF mRNA compared with negative control. Therefore, the disease-free survival of all patients after curative resection can be considered in association with all three markers expression.
我们采用免疫组织化学方法研究了HIF-1、CXCR4和VEGF表达在人类结肠癌中的临床病理意义。HIF-1、CXCR4和VEGF的高表达水平与TNM分期、淋巴结受累及远处转移呈正相关。此外，我们发现这三种分子中任意两种的联合高表达（HIF-1/CXCR4，P = 0.028；HIF-1/VEGF，P = 0.007；CXCR4/VEGF，P = 0.004）与淋巴结转移的相关性比单一分子高表达更强。然而，与单一分子高表达相比，仅CXCR4/VEGF组合与远处转移有关（HIF-1/CXCR4，P = 0.069；HIF-1/VEGF，P = 0.062；CXCR4/VEGF，P = 0.035）。三种分子的联合表达与淋巴结转移和远处转移密切相关。通过实时PCR对不同结肠癌组织样本中HIF-1、CXCR4和VEGF的mRNA表达进行定量，实验结果显示，与阴性对照相比，新鲜结肠组织样本中CXCR4和VEGF mRNA显著过表达。因此，所有患者根治性切除后的无病生存期可与这三种标志物的表达相关联来考虑。
