College of Environmental Science and Engineering, Hunan University, Changsha 410082, People's Republic of China.
Appl Microbiol Biotechnol. 2011 Mar;89(5):1637-44. doi: 10.1007/s00253-010-2927-2. Epub 2010 Oct 16.
Valuable endophytic strains facilitating plants growth and detoxification of heavy metals are required because the application of plant-endophyte symbiotic system is a promising potential technique to improve efficiency of phytoremediation. In this study, endophytic bacterium LRE07 was isolated from cadmium hyperaccumulator Solanum nigrum L. It was identified as Serratia sp. by 16S rRNA sequence analysis. The endophytic bacterium LRE07 was resistant to the toxic effects of heavy metals, solubilized mineral phosphate, and produced indoleacetic acid and siderophore. The heavy metal detoxification was studied in growing LRE07 cells. The strain bound over 65% of cadmium and 35% of zinc in its growing cells from single metal solutions 72 h after inoculation. Besides the high removal efficiencies in single-ion system, an analogous removal phenomenon was also observed in multi-ions system, indicating that the endophyte possesses specific and remarkable heavy metal remediation abilities.
需要具有促进植物生长和重金属解毒功能的有价值内生菌株，因为植物-内生菌共生系统的应用是提高植物修复效率的一种很有前途的潜在技术。在本研究中，从镉超积累植物龙葵中分离出内生细菌 LRE07，通过 16S rRNA 序列分析鉴定为沙雷氏菌属。内生细菌 LRE07 能够抵抗重金属的毒性作用，溶解矿物磷酸盐，并产生吲哚乙酸和铁载体。研究了生长中的 LRE07 细胞对重金属的解毒作用。在接种后 72 小时，该菌株从单一金属溶液中结合了超过 65%的镉和 35%的锌。除了在单离子系统中具有高去除效率外，在多离子系统中也观察到类似的去除现象，表明该内生菌具有特定和显著的重金属修复能力。
