Aravind R, Kumar A, Eapen S J, Ramana K V
Indian Institute of Spices Research, Calicut, Kerala, India.
Lett Appl Microbiol. 2009 Jan;48(1):58-64. doi: 10.1111/j.1472-765X.2008.02486.x. Epub 2008 Nov 14.
To isolate and identify black pepper (Piper nigrum L) associated endophytic bacteria antagonistic to Phytophthora capsici causing foot rot disease.
Endophytic bacteria (74) were isolated, characterized and evaluated against P. capsici. Six genera belong to Pseudomonas spp (20 strains), Serratia (1 strain), Bacillus spp. (22 strains), Arthrobacter spp. (15 strains), Micrococcus spp. (7 strains), Curtobacterium sp. (1 strain) and eight unidentified strains were isolated from internal tissues of root and stem. Three isolates, IISRBP 35, IISRBP 25 and IISRBP 17 were found effective for Phytophthora suppression in multilevel screening assays which recorded over 70% disease suppression in greenhouse trials. A species closest match (99% similarity) of IISRBP 35 was established as Pseudomonas aeruginosa (Pseudomonas EF568931), IISRBP 25 as P. putida (Pseudomonas EF568932), and IISRBP 17 as Bacillus megaterium (B. megaterium EU071712) based on 16S rDNA sequencing.
Black pepper associated P. aeruginosa, P. putida and B. megaterium were identified as effective antagonistic endophytes for biological control of Phytophthora foot rot in black pepper.
This work provides the first evidence for endophytic bacterial diversity in black pepper stem and roots, with biocontrol potential against P. capsici infection.
分离并鉴定与黑胡椒（Piper nigrum L）相关的、对引起根腐病的辣椒疫霉具有拮抗作用的内生细菌。
分离、鉴定了74株内生细菌，并对其进行了抗辣椒疫霉的评估。从根和茎的内部组织中分离出六个属的细菌，分别是假单胞菌属（20株）、沙雷氏菌属（1株）、芽孢杆菌属（22株）、节杆菌属（15株）、微球菌属（7株）、短小杆菌属（1株）以及八株未鉴定的菌株。在多级筛选试验中，发现三株分离株IISRBP 35、IISRBP 25和IISRBP 17对辣椒疫霉具有抑制作用，在温室试验中其病害抑制率超过70%。基于16S rDNA测序，确定与IISRBP 35最相似的物种（相似度99%）为铜绿假单胞菌（假单胞菌EF568931），IISRBP 25为恶臭假单胞菌（假单胞菌EF568932），IISRBP 17为巨大芽孢杆菌（巨大芽孢杆菌EU071712）。
与黑胡椒相关的铜绿假单胞菌、恶臭假单胞菌和巨大芽孢杆菌被鉴定为对黑胡椒根腐病具有生物防治作用的有效拮抗内生菌。
本研究首次提供了黑胡椒茎和根中内生细菌多样性的证据，这些内生细菌具有对辣椒疫霉感染的生物防治潜力。
