The Laboratory of Molecular Genetics and Breeding of Yeast, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Biotechnol Lett. 2011 Feb;33(2):277-84. doi: 10.1007/s10529-010-0433-3. Epub 2010 Oct 16.
The FPS1 gene coding for the Fps1p aquaglyceroporin protein of an industrial strain of Saccharomyces cerevisiae was disrupted by inserting CUP1 gene. Wild-type strain, CE25, could only grow on YPD medium containing less than 0.45% (v/v) acetic acid, while recombinant strain T12 with FPS1 disruption could grow on YPD medium with 0.6% (v/v) acetic acid. Under 0.4% (v/v) acetic acid stress (pH 4.26), ethanol production and cell growth rates of T12 were 1.7 ± 0.1 and 0.061 ± 0.003 g/l h, while those of CE25 were 1.2 ± 0.1 and 0.048 ± 0.003 g/l h, respectively. FPS1 gene disruption in an industrial ethanologenic yeast thus increases cell growth and ethanol yield under acetic acid stress, which suggests the potential utility of FPS1 gene disruption for bioethanol production from renewable resources such as lignocelluloses.
工业酿酒酵母菌株的 FPS1 基因被插入 CUP1 基因破坏，该基因编码 Fps1p 水甘油通道蛋白。野生型菌株 CE25 只能在含有小于 0.45%（v/v）乙酸的 YPD 培养基中生长，而具有 FPS1 破坏的重组菌株 T12 可以在含有 0.6%（v/v）乙酸的 YPD 培养基中生长。在 0.4%（v/v）乙酸胁迫（pH 4.26）下，T12 的乙醇产量和细胞生长速率分别为 1.7±0.1 和 0.061±0.003 g/l h，而 CE25 的分别为 1.2±0.1 和 0.048±0.003 g/l h。因此，在工业乙醇生产酵母中破坏 FPS1 基因可提高细胞在乙酸胁迫下的生长和乙醇产量，这表明破坏 FPS1 基因可能有助于利用可再生资源（如木质纤维素）生产生物乙醇。
