卵丘细胞中表达的人类卵母细胞发育能力的生物标志物：一项初步研究。
Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study.
机构信息
Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, Ste-Foy, Québec, Canada, G1K 7P4.
出版信息
J Assist Reprod Genet. 2011 Feb;28(2):173-88. doi: 10.1007/s10815-010-9491-7. Epub 2010 Oct 16.
PURPOSE
To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria.
METHODS
Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intra-patient analysis were performed on 17 selected candidates.
RESULTS
Seven genes were differentially (p ≤ 0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence.
CONCLUSIONS
These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection. Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency.
目的
鉴定在卵丘细胞中表达的可靠基因组生物标志物，这些生物标志物能够准确、无创地预测卵母细胞的发育能力，并强化已有的形态学标准。
方法
选择 8 名同意进行卵巢刺激和卵胞浆内单精子注射（ICSI）程序的患者。经阴道穿刺卵丘-卵母细胞复合体，并根据良好的形态学标准和高透明带双折射，对其进行单独选择。ICSI 后，每个患者移植两个 3 天的胚胎。记录妊娠结局，并随后确认证实的着床。使用两个微阵列平台评估差异基因表达。对 17 名选定的候选人进行进一步的实时 PCR 验证、Ingenuity 途径分析和患者内分析。
结果
有 7 个基因与成功妊娠和着床显著相关（p≤0.05）。这些生物标志物可用于预测卵母细胞的发育能力。
结论
这些基因组标记物是对卵母细胞选择的形态学方法的有力强化。对其进行大规模验证可能会提高妊娠结局和单胚胎移植的效率。
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