Institute of Reproductive Health and Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, P. R. China.
Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, P. R. China.
Syst Biol Reprod Med. 2020 Jun;66(3):176-184. doi: 10.1080/19396368.2020.1725685. Epub 2020 Mar 6.
To study the relationship between the expression of 10 selected genes in cumulus cells and the corresponding oocyte development competence, and the effect of patient age and body mass index on gene expression of cumulus cells, we collected 354 cumulus cell masses associated with individual oocyte from 48 women. The expression levels of the genes involved in glucose metabolism ( and ) and expansion () in cumulus cells were detected by reverse transcription polymerase chain reaction. These were compared among oocyte maturity, fertilization, embryo morphology and implantation, and analyzed the effect of the subject's age and body mass index. Cumulus cell expression from mature oocytes was higher than those from immature oocytes (P = 0.014), and expression was higher from oocytes that developed into high-quality embryos (P = 0.024). expression in cumulus cells from fertilized oocytes was lower than that from unfertilized oocytes (P = 0.044). The levels of and were changed significantly as a function of the subject's age and body mass index. In conclusion, the level of expression in cumulus cells is positively correlated with the early embryo morphology score, and with further development could perhaps be used to evaluate oocyte developmental competence to complement embryonic morphological assessment.
CCs: cumulus cells; GDF9: growth differentiation factor 9; BMP15: bone morphogenetic protein 15; PTGS2: prostaglandin synthase 2; HAS2: hyaluronic acid synthase 2; VCAN: versican; GREM1: gremlin 1; PFKP: phosphofructokinase, platelet; PKM2: pyruvate kinase isozyme type M2; LDHA: lactic dehydrogenase; GFPT: glucosaminefructo-6-phosphate transaminase; TNFAIP6: tumor necrosis factor 6 protein; PTX3: penetrin 3; SDC4: syndecan-4; BMI: body mass index; MD: median values; IQR: interquartile range; FSH: follicle-stimulating hormone; LH: luteinizing hormone; HCG: human chorionic gonadotropin; ICSI: intracytoplasmic sperm injection; GnRH: gonadotropin-releasing hormone; hMG: human menopausal gonadotropin; GV: germinal vesicle; M I: metaphase I; M II: metaphase II; cDNA: complementary DNA; SD: standard deviation.
研究卵丘细胞中 10 个选定基因的表达与相应卵母细胞发育能力之间的关系，以及患者年龄和体重指数对卵丘细胞基因表达的影响，我们收集了 48 名妇女的 354 个与单个卵母细胞相关的卵丘细胞团。通过逆转录聚合酶链反应检测卵丘细胞中参与葡萄糖代谢的基因（和）和扩张（）的表达水平。比较卵母细胞成熟度、受精、胚胎形态和着床情况，并分析受试者年龄和体重指数的影响。成熟卵母细胞的卵丘细胞表达高于不成熟卵母细胞（P = 0.014），高质量胚胎发育的卵母细胞表达更高（P = 0.024）。受精卵母细胞的卵丘细胞表达低于未受精卵母细胞（P = 0.044）。和的水平随受试者年龄和体重指数的变化而显著变化。结论：卵丘细胞中表达水平与早期胚胎形态评分呈正相关，随着进一步发展，可能用于评估卵母细胞发育能力，以补充胚胎形态评估。
CCs：卵丘细胞；GDF9：生长分化因子 9；BMP15：骨形态发生蛋白 15；PTGS2：前列腺素合酶 2；HAS2：透明质酸合酶 2；VCAN：软骨素 1；GREM1：gremlin 1；PFKP：血小板磷酸果糖激酶；PKM2：丙酮酸激酶同工酶 M2；LDHA：乳酸脱氢酶；GFPT：氨基葡萄糖-果糖-6-磷酸转氨酶；TNFAIP6：肿瘤坏死因子 6 蛋白；PTX3：穿透素 3；SDC4：连接蛋白 4；BMI：体重指数；MD：中位数；IQR：四分位距；FSH：卵泡刺激素；LH：黄体生成素；HCG：人绒毛膜促性腺激素；ICSI：胞浆内精子注射；GnRH：促性腺激素释放激素；hMG：人绝经促性腺激素；GV：生发泡；MI：第一次减数分裂；M II：第二次减数分裂；cDNA：互补 DNA；SD：标准差。
