Institute of Molecular Biology and Biotechnology, FO.R.T.H, 71110 Heraklion, Greece.
Eur Biophys J. 2011 Feb;40(2):209-15. doi: 10.1007/s00249-010-0632-9. Epub 2010 Oct 17.
The effect of the cell glycocalyx on the binding of a membrane receptor, class I major histocompatibility complex (MHC) human leukocyte antigen (HLA)-A2, to an immobilized anti-HLA antibody was investigated using an acoustic sensor based on a Love wave geometry. The enzyme neuraminidase was used to remove sialic acid residues from the cell glycocalyx. Real-time measurements of the amplitude of the acoustic wave showed that treatment with neuraminidase facilitates HLA/anti-HLA-mediated cell attachment via a 3.6-fold increase of the two-dimensional (2D) binding constant of the interaction. This could be attributed to better approach of binding partners due to favorable condition of the desialylated glycocalyx. The results underline the importance of microtopological factors in membrane receptor binding and reveal the potential of the Love wave sensor and 2D binding parameters for studying cell-substrate binding events.
使用基于声表面波（Love 波）几何形状的声学传感器研究了细胞糖萼对膜受体（I 类主要组织相容性复合体（MHC）人类白细胞抗原（HLA）-A2）与固定化抗 HLA 抗体结合的影响。使用神经氨酸酶去除细胞糖萼中的唾液酸残基。声波幅度的实时测量表明，通过增加二维（2D）相互作用的结合常数来促进 HLA/抗 HLA 介导的细胞附着，这是由于去唾液酸化糖萼的有利条件使配体更易于接近。结果强调了微拓扑因素在膜受体结合中的重要性，并揭示了 Love 波传感器和 2D 结合参数在研究细胞-底物结合事件中的潜力。
