Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China.
Sci China Life Sci. 2010 Oct;53(10):1189-95. doi: 10.1007/s11427-010-4070-9. Epub 2010 Oct 17.
Elucidating the underlying mechanisms of cell physiology is currently an important research topic in life sciences. Atomic force microscopy methods can be used to investigate these molecular mechanisms. In this study, single-molecule force spectroscopy was used to explore the specific recognition between the CD20 antigen and anti-CD20 antibody Rituximab on B lymphoma cells under near-physiological conditions. The CD20-Rituximab specific binding force was measured through tip functionalization. Distribution of CD20 on the B lymphoma cells was visualized three-dimensionally. In addition, the relationship between the intramolecular force and the molecular extension of the CD20-Rituximab complex was analyzed under an external force. These results facilitate further investigation of the mechanism of Rituximab's anti-cancer effect.
阐明细胞生理学的潜在机制是当前生命科学的一个重要研究课题。原子力显微镜方法可用于研究这些分子机制。在这项研究中，采用单分子力谱技术，在近生理条件下研究了 B 淋巴瘤细胞上的 CD20 抗原和抗 CD20 抗体利妥昔单抗之间的特异性识别。通过尖端功能化测量 CD20-Rituximab 的特异性结合力。三维可视化 B 淋巴瘤细胞上 CD20 的分布。此外，还分析了在外部力作用下 CD20-Rituximab 复合物的分子伸展与分子内力之间的关系。这些结果有助于进一步研究利妥昔单抗抗癌作用的机制。
