Li Mi, Xiao Xiubin, Liu Lianqing, Xi Ning, Wang Yuechao, Dong Zaili, Zhang Weijing
State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China.
Scanning. 2013 Jan-Feb;35(1):40-6. doi: 10.1002/sca.21033. Epub 2012 Aug 13.
Atomic force microscopy (AFM) provides a new technology to visualize the cellular topography and quantify the molecular interactions at nanometer spatial resolution. In this work, AFM was used to image the cellular topography and measure the molecular force of pathological cells from B-cell lymphoma patients. After the fluorescence staining, cancer cells were recognized by their special morphological features and then the detailed topography was visualized by AFM imaging. The AFM images showed that cancer cells were much rougher than healthy cells. CD20 is a surface marker of B cells and rituximab is a monoclonal antibody against CD20. To measure the CD20-rituximab interaction forces, the polyethylene glycol (PEG) linker was used to link rituximab onto the AFM tip and the verification experiments of the functionalized probe indicated that rituximab molecules were successfully linked onto the AFM tip. The CD20-rituximab interaction forces were measured on about 20 pathological cells and the force measurement results indicated the CD20-rituximab binding forces were mainly in the range of 110-120 pN and 130-140 pN. These results can improve our understanding of the topography and molecular force of lymphoma pathological cells.
原子力显微镜（AFM）提供了一种新技术，可在纳米空间分辨率下可视化细胞形貌并量化分子相互作用。在这项工作中，AFM被用于对B细胞淋巴瘤患者的病理细胞进行细胞形貌成像和测量分子力。荧光染色后，癌细胞通过其特殊的形态特征被识别出来，然后通过AFM成像可视化详细的形貌。AFM图像显示癌细胞比健康细胞粗糙得多。CD20是B细胞的表面标志物，利妥昔单抗是一种针对CD20的单克隆抗体。为了测量CD20-利妥昔单抗的相互作用力，使用聚乙二醇（PEG）连接子将利妥昔单抗连接到AFM探针尖端，功能化探针的验证实验表明利妥昔单抗分子成功连接到AFM探针尖端。在约20个病理细胞上测量了CD20-利妥昔单抗的相互作用力，力测量结果表明CD20-利妥昔单抗的结合力主要在110-120 pN和130-140 pN范围内。这些结果可以增进我们对淋巴瘤病理细胞的形貌和分子力的理解。
