Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea.
Trop Med Int Health. 2010 Dec;15(12):1436-41. doi: 10.1111/j.1365-3156.2010.02657.x. Epub 2010 Oct 18.
Plasmodium vivax malaria is the indigenous strain in the Republic of Korea (ROK). Plasmodium vivax can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood-related products in ROK. We evaluated a P. vivax-specific enzyme-linked immunosorbent assay (Genedia Malaria Ab ELISA 2.0, Green Cross, ROK) with blood samples from four groups: 251 samples from P. vivax-infected patients, 39 samples from post-treatment patients upon follow-up, 200 samples from healthy volunteers and 421 samples from domestic travellers to and from high endemic areas of ROK. The positive cases from the ELISA test were confirmed by both Giemsa microscopic and polymerase chain reaction methods. The clinical sensitivity and specificity of detecting P. vivax with ELISA test were 94.4% and 99.0%, respectively. Thirteen of 421 domestic travellers (3.0%) to endemic areas tested positive. The results indicate the effectiveness of detecting antibodies against P. vivax in blood with Genedia Malaria Ab ELISA 2.0 test in a large blood screen setting.
卵形疟原虫疟疾是大韩民国（ROK）的本土菌株。卵形疟原虫可以通过各种血液成分的输血传播，这成为 ROK 输血和血液制品安全的严重问题。我们评估了一种卵形疟原虫特异性酶联免疫吸附试验（Genedia Malaria Ab ELISA 2.0，Green Cross，ROK），使用了来自四组的血液样本：251 份来自感染卵形疟原虫的患者，39 份来自治疗后随访的患者，200 份来自健康志愿者，421 份来自往返于 ROK 高度流行地区的国内旅行者。ELISA 检测的阳性病例通过吉姆萨显微镜和聚合酶链反应方法得到确认。ELISA 检测卵形疟原虫的临床灵敏度和特异性分别为 94.4%和 99.0%。在 421 名前往流行地区的国内旅行者中，有 13 人（3.0%）检测呈阳性。结果表明，在大规模血液筛查中，Genedia Malaria Ab ELISA 2.0 试验检测血液中针对卵形疟原虫的抗体是有效的。
