Oh Jun Seo, Kim Jang Su, Lee Chang Hwan, Nam Deok Hwa, Kim Sun Hyung, Park Dae Won, Lee Chang Kyu, Lim Chae Seung, Park Gil Hong
Laboratory of Cellular Oncology, Department of Laboratory Medicine, College of Medicine, Korea University Ansan Hospital, Gojan 1-dong, Danwon gu, Ansan, Gyeonggi do 425-707, Republic of Korea .
Mem Inst Oswaldo Cruz. 2008 Feb;103(1):75-8. doi: 10.1590/s0074-02762008005000008. Epub 2008 Jan 31.
Transfusion-transmitted malaria is rare, but it may produce severe problem in the safety of blood transfusion due to the lack of reliable procedure to evaluate donors potentially exposed to malaria. Here, we evaluated a new enzyme-linked immunosorbent assay malaria antibody test (ELISA malaria antibody test, DiaMed, Switzerland) to detect antibodies to Plasmodium vivax (the indigenous malaria) in the blood samples in the Republic of Korea (ROK). Blood samples of four groups were obtained and analyzed; 100 samples from P.vivax infected patients, 35 from recovery patients, 366 from normal healthy individuals, and 325 from domestic travelers of non-endemic areas residents to risky areas of ROK. P.vivax antibody levels by ELISA were then compared to the results from microscopic examination and polymerase chain reaction (PCR) test. As a result, the ELISA malaria antibody test had a clinical sensitivity of 53.0% and a clinical specificity of 94.0% for P.vivax. Twenty out of 325 domestic travelers (6.2%) were reactive and 28 cases (8.6%) were doubtful. Of the reactive and doubtful cases, only two were confirmed as acute malaria by both microscopy and PCR test. Thus we found that the ELISA malaria antibody test was insufficiently sensitive for blood screening of P.vivax in ROK.
输血传播的疟疾很罕见，但由于缺乏评估潜在接触疟疾供血者的可靠程序，它可能会给输血安全带来严重问题。在此，我们评估了一种新的酶联免疫吸附试验疟疾抗体检测（ELISA疟疾抗体检测，DiaMed，瑞士），以检测韩国血液样本中针对间日疟原虫（本土疟疾）的抗体。获取并分析了四组血液样本；100份来自间日疟原虫感染患者，35份来自康复患者，366份来自正常健康个体，325份来自非流行地区居民前往韩国危险地区的国内旅行者。然后将ELISA检测的间日疟原虫抗体水平与显微镜检查和聚合酶链反应（PCR）检测结果进行比较。结果，ELISA疟疾抗体检测对间日疟原虫的临床敏感性为53.0%，临床特异性为94.0%。325名国内旅行者中有20名（6.2%）呈反应性，28例（8.6%）为可疑。在反应性和可疑病例中，只有两例通过显微镜检查和PCR检测均被确认为急性疟疾。因此，我们发现ELISA疟疾抗体检测在韩国对间日疟原虫血液筛查的敏感性不足。
